{"doi":"10.1104/pp.125.2.711","title":"Molecular Interactions between the Specialist Herbivore<i>Manduca sexta</i> (Lepidoptera, Sphingidae) and Its Natural Host <i>Nicotiana attenuata</i>. III. Fatty Acid-Amino Acid Conjugates in Herbivore Oral Secretions Are Necessary and Sufficient for Herbivore-Specific Plant Responses","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Feeding by the tobacco specialist Manduca sexta(Lepidoptera, Sphingidae) and application of larval oral secretions and regurgitant (R) to mechanical wounds are known to elicit: (a) a systemic release of mono- and sesquiterpenes, (b) a jasmonate burst, and (c) R-specific changes in transcript accumulation of putatively growth- and defense-related mRNAs in Nicotiana attenuata Torr. ex Wats. We identified several fatty acid-amino acid conjugates (FACs) in the R of M. sexta and the closely related species Manduca quinquemaculata which, when synthesized and applied to mechanical wounds at concentrations comparable with those found in R, elicited all three R-specific responses. Ion-exchange treatment of R, which removed all detectable FACs and free fatty acids (FAs), also removed all detectable activity. The biological activity of ion-exchanged R could be completely restored by the addition of synthetic FACs at R-equivalent concentrations, whereas the addition of FAs did not restore the biological activity of R. We conclude that the biological activity of R is not related to the supply of FAs to the octadecanoid cascade for endogenous jasmonate biosynthesis, but that FACs elicit the herbivore-specific responses by another mechanism and that the insect-produced modification of plant-derived FAs is necessary for the plant's recognition of this specialized herbivore.</jats:p>","journal":"Plant Physiology","year":2001,"id":32441,"datarank":12.995954996033019,"base_score":6.234410725718371,"endowment":6.234410725718371,"self_citation_contribution":0.9351616088577558,"citation_network_contribution":12.060793387175263,"self_endowment_contribution":0.9351616088577558,"citer_contribution":12.060793387175263,"corpus_percentile":null,"corpus_rank":null,"citation_count":509,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":171566,"name":"Ursula Schittko","orcid":null,"position":1,"is_corresponding":false},{"id":171714,"name":"Georg Pohnert","orcid":null,"position":2,"is_corresponding":false},{"id":145712,"name":"Wilhelm Boland","orcid":null,"position":3,"is_corresponding":false},{"id":158961,"name":"Ian T. Baldwin","orcid":"0000-0001-5371-2974","position":4,"is_corresponding":false},{"id":171713,"name":"Rayko Halitschke","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":6.234410725718371,"endowment":6.234410725718371,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11161028","pmcid":null,"openalex_id":"https://openalex.org/W2152410263","authors":[],"funders":[],"total_grants":0,"fwci":42.5787,"citation_percentile":0.99953655,"influential_citations":17,"citation_trend":[{"year":2012,"count":37},{"year":2013,"count":21},{"year":2014,"count":23},{"year":2015,"count":17},{"year":2016,"count":14},{"year":2017,"count":17},{"year":2018,"count":15},{"year":2019,"count":11},{"year":2020,"count":14},{"year":2021,"count":24},{"year":2022,"count":9},{"year":2023,"count":6},{"year":2024,"count":12},{"year":2025,"count":5},{"year":2026,"count":1}],"oa_status":"bronze","license":"https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model","oa_locations":[{"url":"https://academic.oup.com/plphys/article-pdf/125/2/711/38678081/plphys_v125_2_711.pdf","host_type":"journal"},{"url":"https://academic.oup.com/plphys/article-pdf/125/2/711/38678081/plphys_v125_2_711.pdf","host_type":"BRONZE"},{"url":"https://academic.oup.com/plphys/article-pdf/125/2/711/38678081/plphys_v125_2_711.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/plphys/article-pdf/125/2/711/38678081/plphys_v125_2_711.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1104/pp.125.2.711","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11161028","host_type":"repository"},{"url":"http://infoscience.epfl.ch/record/78358","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/64872","host_type":"repository"}],"fields_of_study":["Insect-Plant Interactions and Control","Insect and Pesticide Research","Insect Pest Control Strategies","Biology","Medicine","Environmental Science","Amino Acids","Animals","Bridged Bicyclo Compounds","Cyclopentanes","Fatty Acids, Nonesterified","Fatty Acids, Volatile","Host-Parasite Interactions","Manduca","Molecular Sequence Data","Oxylipins","Nicotiana","Transcription, Genetic"],"mesh_terms":["Amino Acids","Animals","Bridged Bicyclo Compounds","Cyclopentanes","Fatty Acids, Nonesterified","Fatty Acids, Volatile","Host-Parasite Interactions","Molecular Sequence Data","Nicotiana","Transcription, Genetic","Manduca","Oxylipins"],"keywords":["Manduca sexta","Sphingidae","Manduca","Biology","Biochemistry","Methyl jasmonate","Lepidoptera genitalia","Nicotiana tabacum","Jasmonate","Nicotiana","Insect","Fatty acid","Solanaceae","Biosynthesis","Plant defense against herbivory","Botany","Enzyme","Gene","Arabidopsis"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.16544334.v1","title":"Additional file 1 of Light dominates the diurnal emissions of herbivore-induced volatiles in wild tobacco","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.16544334","title":"Additional file 1 of Light dominates the diurnal emissions of herbivore-induced volatiles in wild tobacco","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T12:37:48.331981Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}