{"doi":"10.1104/pp.117.2.545","title":"Strontium-Induced Repetitive Calcium Spikes in a Unicellular Green Alga1","abstract":"<jats:title>Abstract</jats:title><jats:p>The divalent cation Sr2+ induced repetitive transient spikes of the cytosolic Ca2+ activity [Ca2+]cy and parallel repetitive transient hyperpolarizations of the plasma membrane in the unicellular green algaEremosphaera viridis. [Ca2+]cymeasurements, membrane potential measurements, and cation analysis of the cells were used to elucidate the mechanism of Sr2+-induced [Ca2+]cyoscillations. Sr2+ was effectively and rapidly compartmentalized within the cell, probably into the vacuole. The [Ca2+]cy oscillations cause membrane potential oscillations, and not the reverse. The endoplasmic reticulum (ER) Ca2+-ATPase blockers 2,5-di-tert-butylhydroquinone and cyclopiazonic acid inhibited Sr2+-induced repetitive [Ca2+]cy spikes, whereas the compartmentalization of Sr2+ was not influenced. A repetitive Ca2+ release and Ca2+ re-uptake by the ER probably generated repetitive [Ca2+]cyspikes in E. viridis in the presence of Sr2+. The inhibitory effect of ruthenium red and ryanodine indicated that the Sr2+-induced Ca2+ release from the ER was mediated by a ryanodine/cyclic ADP-ribose type of Ca2+ channel. The blockage of Sr2+-induced repetitive [Ca2+]cy spikes by La3+ or Gd3+ indicated the necessity of a certain influx of divalent cations for sustained [Ca2+]cy oscillations. Based on these data we present a mathematical model that describes the baseline spiking [Ca2+]cy oscillations in E. viridis.</jats:p>","journal":"Plant Physiology","year":1998,"id":24646,"datarank":3.477156160526812,"base_score":4.04305126783455,"endowment":4.04305126783455,"self_citation_contribution":0.6064576901751826,"citation_network_contribution":2.8706984703516296,"self_endowment_contribution":0.6064576901751826,"citer_contribution":2.8706984703516296,"corpus_percentile":null,"corpus_rank":null,"citation_count":56,"citer_count":48,"citers_with_citation_signal":47,"citers_with_endowment":47,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":147291,"name":"Christoph Plieth","orcid":null,"position":1,"is_corresponding":false},{"id":147292,"name":"Birgit Bethmann","orcid":null,"position":2,"is_corresponding":false},{"id":147293,"name":"Ondina Popescu","orcid":null,"position":3,"is_corresponding":false},{"id":147294,"name":"Ulf-Peter Hansen","orcid":null,"position":4,"is_corresponding":false},{"id":147295,"name":"Wilhelm Simonis","orcid":null,"position":5,"is_corresponding":false},{"id":147296,"name":"Gerald Schönknecht","orcid":null,"position":6,"is_corresponding":false},{"id":147290,"name":"Claudia S. Bauer","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.04305126783455,"endowment":4.04305126783455,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9625707","pmcid":null,"openalex_id":"https://openalex.org/W2151872223","authors":[],"funders":[],"total_grants":0,"fwci":2.4516,"citation_percentile":0.9004244,"influential_citations":4,"citation_trend":[{"year":2013,"count":3},{"year":2015,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1}],"oa_status":"bronze","license":"https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model","oa_locations":[{"url":"https://academic.oup.com/plphys/article-pdf/117/2/545/37215131/plphys_v117_2_545.pdf","host_type":"journal"},{"url":"https://academic.oup.com/plphys/article-pdf/117/2/545/37215131/plphys_v117_2_545.pdf","host_type":"BRONZE"},{"url":"https://academic.oup.com/plphys/article-pdf/117/2/545/37215131/plphys_v117_2_545.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/plphys/article-pdf/117/2/545/37215131/plphys_v117_2_545.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1104/pp.117.2.545","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9625707","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/34974","host_type":"repository"}],"fields_of_study":["Photoreceptor and optogenetics research","Photosynthetic Processes and Mechanisms","Ion channel regulation and function","Medicine","Biology","Environmental Science"],"mesh_terms":[],"keywords":["Ruthenium red","Cyclopiazonic acid","Ryanodine receptor","Divalent","Endoplasmic reticulum","Chemistry","Biophysics","Calcium","Cytosol","Biochemistry","Biology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T22:54:33.365496Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}