{"doi":"10.1104/pp.109.3.1039","title":"Expression of Functional Oat Phytochrome A in Transgenic Rice","abstract":null,"journal":"Plant Physiology","year":1995,"id":677506,"datarank":0.5641800173540344,"base_score":3.7612001156935624,"endowment":3.7612001156935624,"self_citation_contribution":0.5641800173540344,"citation_network_contribution":0.0,"self_endowment_contribution":0.5641800173540344,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":42,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1732751,"name":"J. J. Casal","orcid":null,"position":1,"is_corresponding":false},{"id":1770241,"name":"E. T. Jordan","orcid":null,"position":2,"is_corresponding":false},{"id":1750047,"name":"P. Christou","orcid":null,"position":3,"is_corresponding":false},{"id":1770242,"name":"R. D. Vierstra","orcid":null,"position":4,"is_corresponding":false},{"id":1770239,"name":"R. C. Clough","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Expression of Functional Oat Phytochrome A in Transgenic Rice","abstract":"To investigate the biological functions of phytochromes in monocots, we generated, by electric discharge particle bombardment, transgenic rice (Oryza sativa cv Gulfmont) that constitutively expresses the oat phytochrome A apoprotein. The introduced 124-kD polypeptide bound chromophore and assembled into a red- and far-red-light-photoreversible chromoprotein with absorbance spectra indistinguishable from those of phytochrome purified from etiolated oats. Transgenic lines expressed up to 3 and 4 times more spectrophotometrically detectable phytochrome than wild-type plants in etiolated and green seedlings, respectively. Upon photo-conversion to the far-red-absorbing form of phytochrome, oat phytochrome A was degraded in etiolated seedlings with kinetics similar to those of endogenous rice phytochromes (half-life approximately 20 min). Although plants overexpressing phytochrome A were phenotypically indistinguishable from wild-type plants when grown under high-fluence white light, they were more sensitive as etiolated seedlings to light pulses that established very low phytochrome equilibria. This indicates that the introduced oat phytochrome A was biologically active. Thus, rice ectopically expressing PHY genes may offer a useful model to help understand the physiological functions of the various phytochrome isoforms in monocotyledonous plants.","is_dataset_classified":null,"base_score":3.7612001156935624,"endowment":3.7612001156935624,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8552709","pmcid":null,"openalex_id":"https://openalex.org/W2085167228","authors":[],"funders":[],"total_grants":0,"fwci":4.2009,"citation_percentile":0.93621574,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":3},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2017,"count":2},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2026,"count":1}],"oa_status":"bronze","license":"http://aspb.org/publications/aspb-journals/open-articles","oa_locations":[{"url":"https://academic.oup.com/plphys/article-pdf/109/3/1039/35533545/plphys_v109_3_1039.pdf","host_type":"journal"},{"url":"https://academic.oup.com/plphys/article-pdf/109/3/1039/35533545/plphys_v109_3_1039.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1104/pp.109.3.1039","host_type":"publisher"},{"url":"https://doi.org/10.1104/pp.109.3.1039","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8552709","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/161407","host_type":"repository"}],"fields_of_study":["Light effects on plants","Plant and Biological Electrophysiology Studies","Magnetic and Electromagnetic Effects"],"mesh_terms":["Kinetics","Light","Phenotype","Phytochrome","Oryza","Spectrophotometry","Immunoblotting","Genes, Plant","Plant Stems","Cotyledon","Avena","Plants, Genetically Modified","Phytochrome A"],"keywords":["Phytochrome","Etiolation","Phytochrome A","Oryza sativa","Biology","Genetically modified rice","Far-red","Transgene","Gene isoform","Coleoptile","Biochemistry","Botany","Mutant","Red light","Genetically modified crops","Arabidopsis","Gene","Enzyme"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T04:13:02.643844Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}