{"doi":"10.1101/pdb.prot108185","title":"High-Throughput Mutant Screening in<i>Vibrio cholerae</i>via Transposon Sequencing","abstract":"<jats:p>Transposon mutagenesis greatly facilitates the study of gene function in microorganisms ranging from viruses to fungi. Traditionally, one would study individual transposon mutants with interesting phenotypes one mutant at a time. Here, we describe methods for the study of tens of thousands of transposon mutants in parallel in the bacterial pathogen<jats:italic>Vibrio cholerae</jats:italic>using transposon-sequencing. The first section outlines methods for making a saturated transposon mutant library. The second section outlines methods for massively parallel sequencing of the transposon junctions. The third section outlines methods for analyzing the sequence data to calculate the fitness contribution of genes.</jats:p>","journal":"Cold Spring Harbor Protocols","year":2023,"id":43870,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":1,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":207574,"name":"Andrew Camilli","orcid":null,"position":1,"is_corresponding":false},{"id":207573,"name":"Jacob Bourgeois","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36931735","pmcid":"PMC10586728","openalex_id":"https://openalex.org/W4327811729","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"R37 AI055058","title":null}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2023,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10586728/pdf/nihms-1937798.pdf","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586728","host_type":"GREEN"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10586728/pdf/nihms-1937798.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/pdb.prot108185","host_type":"publisher"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10586728","host_type":"repository"},{"url":"https://doi.org/10.1101/pdb.prot108185","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36931735","host_type":"repository"}],"fields_of_study":["Vibrio bacteria research studies","Bacteriophages and microbial interactions","Bacterial Genetics and Biotechnology","Medicine","Biology","Environmental Science","Vibrio cholerae","DNA Transposable Elements","Mutagenesis","High-Throughput Nucleotide Sequencing","Gene Library","Mutagenesis, Insertional"],"mesh_terms":["DNA Transposable Elements","Vibrio cholerae","Gene Library","Mutagenesis, Insertional","Mutagenesis","High-Throughput Nucleotide Sequencing"],"keywords":["Transposable element","Transposon mutagenesis","Vibrio cholerae","Sleeping Beauty transposon system","Mutant","Mutagenesis","Biology","Genetics","Computational biology","Genome","Massive parallel sequencing","Gene","Bacteria"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-15T03:59:21.407228Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}