{"doi":"10.1101/gr.6163007","title":"CpG methylation is targeted to transcription units in an invertebrate genome","abstract":"<jats:p>DNA is methylated at the dinucleotide CpG in genomes of a wide range of plants and animals. Among animals, variable patterns of genomic CpG methylation have been described, ranging from undetectable levels (e.g., in <jats:italic>Caenorhabditis elegans</jats:italic>) to high levels of global methylation in the vertebrates. The most frequent pattern in invertebrate animals, however, is mosaic methylation, comprising domains of methylated DNA interspersed with unmethylated domains. To understand the origin of mosaic DNA methylation patterns, we examined the distribution of DNA methylation in the <jats:italic>Ciona intestinalis</jats:italic> genome. Bisulfite sequencing and computational analysis revealed methylated domains with sharp boundaries that strongly colocalize with ∼60% of transcription units. By contrast, promoters, intergenic DNA, and transposons are not preferentially targeted by DNA methylation. Methylated transcription units include evolutionarily conserved genes, whereas the most highly expressed genes preferentially belong to the unmethylated fraction. The results lend support to the hypothesis that CpG methylation functions to suppress spurious transcriptional initiation within infrequently transcribed genes.</jats:p>","journal":"Genome Research","year":2007,"id":38417,"datarank":10.272145320291402,"base_score":5.521460917862246,"endowment":5.521460917862246,"self_citation_contribution":0.8282191376793371,"citation_network_contribution":9.443926182612065,"self_endowment_contribution":0.8282191376793371,"citer_contribution":9.443926182612065,"corpus_percentile":null,"corpus_rank":null,"citation_count":249,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":25,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":190622,"name":"Alastair R.W. 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and DNA Methylation","Cancer-related gene regulation","Genomics and Chromatin Dynamics","Medicine","Biology","0301 basic medicine","03 medical and health sciences","Animals","Ciona intestinalis","CpG Islands","DNA","DNA Methylation","Sequence Analysis, DNA","Transcription, Genetic"],"mesh_terms":["Animals","Ciona intestinalis","DNA","Transcription, Genetic","Sequence Analysis, DNA","CpG Islands","DNA Methylation"],"keywords":["Biology","RNA-Directed DNA Methylation","DNA methylation","Methylated DNA immunoprecipitation","Genetics","Methylation","Illumina Methylation Assay","Epigenetics of physical exercise","CpG site","Differentially methylated regions","Genome","Promoter","Gene","Bisulfite sequencing","Molecular biology","Gene expression","Transcription, Genetic","Animals","CpG Islands","DNA","Sequence Analysis, DNA","Ciona intestinalis"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.13633194.v1","title":"Additional file 1 of The effect of DNA methylation on 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