{"doi":"10.1101/gr.1949704","title":"Two Methods of Whole-Genome Amplification Enable Accurate Genotyping Across a 2320-SNP Linkage Panel","abstract":"<jats:p>Comprehensive genome scans involving many thousands of SNP assays will require significant amounts of genomic DNA from each sample. We report two successful methods for amplifying whole-genomic DNA prior to SNP analysis, multiple displacement amplification, and OmniPlex technology. We determined the coverage of amplification by analyzing a SNP linkage marker set that contained 2320 SNP markers spread across the genome at an average distance of 2.5 cM. We observed a concordance of &gt;99.8% in genotyping results from genomic DNA and amplified DNA, strongly indicating the ability of both methods used to amplify genomic DNA in a highly representative manner. Furthermore, we were able to achieve a SNP call rate of &gt;98% in both genomic and amplified DNA. The combination of whole-genome amplification and comprehensive SNP linkage analysis offers new opportunities for genetic analysis in clinical trials, disease association studies, and archiving of DNA samples.</jats:p>","journal":"Genome Research","year":2004,"id":14994,"datarank":15.15763715208109,"base_score":5.3612921657094255,"endowment":5.3612921657094255,"self_citation_contribution":0.8041938248564139,"citation_network_contribution":14.353443327224676,"self_endowment_contribution":0.8041938248564139,"citer_contribution":14.353443327224676,"corpus_percentile":null,"corpus_rank":null,"citation_count":212,"citer_count":200,"citers_with_citation_signal":174,"citers_with_endowment":174,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":116073,"name":"Mark S.T. 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