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These results suggest that the enzymatic activity and subcellular distribution of c-Src are coordinately regulated during cellular adhesion and that c-Src can affect adhesion by a kinase-independent mechanism.</jats:p>","journal":"Genes &amp; Development","year":1995,"id":635330,"datarank":0.8657161684695025,"base_score":5.771441123130016,"endowment":5.771441123130016,"self_citation_contribution":0.8657161684695025,"citation_network_contribution":0.0,"self_endowment_contribution":0.8657161684695025,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":320,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1648300,"name":"J R Swedlow","orcid":null,"position":1,"is_corresponding":false},{"id":1648301,"name":"D O Morgan","orcid":null,"position":2,"is_corresponding":false},{"id":121341,"name":"H E Varmus","orcid":null,"position":3,"is_corresponding":false},{"id":1648299,"name":"K B Kaplan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"c-Src enhances the spreading of src-/- fibroblasts on fibronectin by a kinase-independent mechanism.","abstract":"<jats:p>We have explored the role of the tyrosine kinase c-Src in cellular adhesion. Fibroblasts derived from src-/- mice (src-/- fibroblasts) exhibit a reduced rate of spreading on fibronectin. These defect is rescued by expression of wild-type chicken c-Src. Analyses of mutants suggest that c-Src increases the rate of cell spreading in src-/- fibroblasts through a kinase-independent mechanism requiring both the SH3 and SH2 domains. To further address the role of c-Src in adhesion, we examined the activity and subcellular distribution of c-Src during the adhesion of fibroblasts on fibronectin. We observed a transient increase in the specific kinase activity of c-Src accompanied by the partial dephosphorylation of the negative regulatory site Y527. Activation of c-Src is followed by its redistribution to newly formed focal adhesions. These results suggest that the enzymatic activity and subcellular distribution of c-Src are coordinately regulated during cellular adhesion and that c-Src can affect adhesion by a kinase-independent mechanism.</jats:p>","is_dataset_classified":null,"base_score":5.771441123130016,"endowment":5.771441123130016,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7541382","pmcid":null,"openalex_id":"https://openalex.org/W1982849870","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2012,"count":8},{"year":2013,"count":8},{"year":2014,"count":3},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":6},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"gold","license":"other-oa","oa_locations":[{"url":"http://genesdev.cshlp.org/content/9/12/1505.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/9/12/1505.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.9.12.1505","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.9.12.1505","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7541382","host_type":"repository"},{"url":"https://discovery.dundee.ac.uk/en/publications/3c3f9967-daba-438c-8a4b-cfd0c0dca938","host_type":"repository"},{"url":"http://www.scopus.com/inward/record.url?scp=0029034939&partnerID=8YFLogxK","host_type":"repository"}],"fields_of_study":["Cell Adhesion Molecules Research","Wnt/β-catenin signaling in development and cancer","Protein Kinase Regulation and GTPase Signaling","Animals","CSK Tyrosine-Protein Kinase","Cell Adhesion","Cell Line, Transformed","Cell Size","Chickens","Fibroblasts","Fibronectins","Mice","Mice, Knockout","Phosphorylation","Phosphotyrosine","Protein Processing, Post-Translational","Protein-Tyrosine Kinases","Proto-Oncogene Proteins pp60(c-src)","Subcellular Fractions","Tyrosine","src-Family Kinases"],"mesh_terms":["CSK Tyrosine-Protein Kinase","Animals","Cell Adhesion","Cell Line, Transformed","Chickens","Fibroblasts","Fibronectins","Phosphorylation","Protein Processing, Post-Translational","Protein-Tyrosine Kinases","Subcellular Fractions","Tyrosine","Proto-Oncogene Proteins pp60(c-src)","Mice, Knockout","Phosphotyrosine","src-Family Kinases","Cell Size","Mice"],"keywords":["Proto-oncogene tyrosine-protein kinase Src","Tyrosine-protein kinase CSK","Focal adhesion","Cell biology","Biology","Fibronectin","SH3 domain","Tyrosine kinase","Cell adhesion","Phosphorylation","PTK2","Signal transduction","Biochemistry","Protein kinase A","Cell","Mitogen-activated protein kinase kinase","Extracellular matrix"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T14:57:10.272709Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}