{"doi":"10.1101/gad.892001","title":"The\n                    <i>lac</i>\n                    operator-repressor system is functional in the mouse","abstract":"<jats:p>\n                    We report the successful transfer of a fully functional\n                    <jats:italic>lac</jats:italic>\n                    operator-repressor gene regulatory system to the mouse. The key component is a\n                    <jats:italic>lac</jats:italic>\n                    repressor transgene that resembles a typical mammalian gene both in codon usage and structure and expresses functional levels of repressor protein in the animal. We used the repressor to regulate the expression of a mammalian reporter gene consisting of the tyrosinase promoter embedded with three short\n                    <jats:italic>lac</jats:italic>\n                    operator sequences and the tyrosinase coding sequence. Pigmentation of the mouse was controlled by the interaction of the\n                    <jats:italic>lac</jats:italic>\n                    repressor with the regulatable\n                    <jats:italic>Tyrosinase</jats:italic>\n                    transgene in a manner that was fully reversible by the lactose analog IPTG. Direct control of mammalian promoters by the\n                    <jats:italic>lac</jats:italic>\n                    repressor provides tight, reversible regulation, predictable levels of de-repressed expression, and the promise of reversible control of the endogenous genome.\n                  </jats:p>","journal":"Genes &amp; Development","year":2001,"id":588566,"datarank":7.094628910383251,"base_score":5.0369526024136295,"endowment":5.0369526024136295,"self_citation_contribution":0.7555428903620446,"citation_network_contribution":6.339086020021206,"self_endowment_contribution":0.7555428903620446,"citer_contribution":6.339086020021206,"corpus_percentile":null,"corpus_rank":null,"citation_count":153,"citer_count":146,"citers_with_citation_signal":135,"citers_with_endowment":135,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1505741,"name":"Wendy Gluba","orcid":null,"position":1,"is_corresponding":false},{"id":1505742,"name":"Heidi Scrable","orcid":null,"position":2,"is_corresponding":false},{"id":1505740,"name":"Carolyn A. Cronin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The\n                    <i>lac</i>\n                    operator-repressor system is functional in the mouse","abstract":"<jats:p>\n                    We report the successful transfer of a fully functional\n                    <jats:italic>lac</jats:italic>\n                    operator-repressor gene regulatory system to the mouse. The key component is a\n                    <jats:italic>lac</jats:italic>\n                    repressor transgene that resembles a typical mammalian gene both in codon usage and structure and expresses functional levels of repressor protein in the animal. We used the repressor to regulate the expression of a mammalian reporter gene consisting of the tyrosinase promoter embedded with three short\n                    <jats:italic>lac</jats:italic>\n                    operator sequences and the tyrosinase coding sequence. Pigmentation of the mouse was controlled by the interaction of the\n                    <jats:italic>lac</jats:italic>\n                    repressor with the regulatable\n                    <jats:italic>Tyrosinase</jats:italic>\n                    transgene in a manner that was fully reversible by the lactose analog IPTG. Direct control of mammalian promoters by the\n                    <jats:italic>lac</jats:italic>\n                    repressor provides tight, reversible regulation, predictable levels of de-repressed expression, and the promise of reversible control of the endogenous genome.\n                  </jats:p>","is_dataset_classified":null,"base_score":5.0369526024136295,"endowment":5.0369526024136295,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11410531","pmcid":"PMC312721","openalex_id":"https://openalex.org/W2158284529","authors":[],"funders":[{"funder_name":"NCRR NIH HHS","grant_id":"RR11102","title":null},{"funder_name":"NCRR NIH HHS","grant_id":"R24 RR011102","title":null},{"funder_name":"NIMH NIH HHS","grant_id":"MH12406","title":null},{"funder_name":"NIMH NIH HHS","grant_id":"F30 MH012406","title":null}],"total_grants":4,"fwci":null,"citation_percentile":null,"influential_citations":16,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":6},{"year":2014,"count":9},{"year":2015,"count":7},{"year":2016,"count":4},{"year":2017,"count":7},{"year":2018,"count":1},{"year":2019,"count":6},{"year":2020,"count":2},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":7},{"year":2024,"count":3},{"year":2025,"count":4}],"oa_status":"gold","license":null,"oa_locations":[{"url":"http://genesdev.cshlp.org/content/15/12/1506.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/15/12/1506.full.pdf","host_type":"GOLD"},{"url":"http://genesdev.cshlp.org/content/15/12/1506.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.892001","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.892001","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11410531","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/312721","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","RNA Interference and Gene Delivery","melanin and skin pigmentation","Biology","Medicine","Animals","Bacterial Proteins","Cell Line","CpG Islands","Escherichia coli Proteins","Gene Expression Regulation","Humans","Isopropyl Thiogalactoside","Lac Operon","Lac Repressors","Mice","Mice, Transgenic","Monophenol Monooxygenase","Operator Regions, Genetic","Protein Biosynthesis","RNA Splicing","Rats","Repressor Proteins","Transcription, Genetic"],"mesh_terms":["Animals","Bacterial Proteins","Cell Line","Gene Expression Regulation","Humans","Isopropyl Thiogalactoside","Lac Operon","Mice, Transgenic","Operator Regions, Genetic","Repressor Proteins","RNA Splicing","Transcription, Genetic","Protein Biosynthesis","Monophenol Monooxygenase","CpG Islands","Escherichia coli Proteins","Mice","Rats","Lac Repressors"],"keywords":["Lac repressor","Repressor","Biology","lac operon","YY1","Operator (biology)","Gene","Transgene","Regulation of gene expression","Promoter","Genetics","Reporter gene","Molecular biology","Regulatory sequence","Gene expression"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-21T19:53:26.015995Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}