{"doi":"10.1101/gad.7.2.180","title":"Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain.","abstract":"<jats:p>TFIID is a multisubunit protein containing the TATA box-binding polypeptide (TBP) and associated factors (TFIID-TAFs) required for activated transcription by RNA polymerase II. TBPs from different eukaryotes contain a highly conserved carboxy-terminal domain and very divergent amino-terminal domains. Earlier studies proposed that the amino-terminal domains of metazoan TBPs are required for activated transcription. However, we report that a human TFIID complex containing an amino-terminal truncated TBP contains all the major TFIID-TAFs and supports in vitro transcriptional stimulation by different classes of activation domains and from a TATA-less promoter. Protein blotting experiments revealed direct interactions between the conserved domain of TBP and the two largest TAFs. The results suggest a model for the interaction of TFIID-TAFs with TBP.</jats:p>","journal":"Genes &amp; Development","year":1993,"id":606194,"datarank":0.6749714505495399,"base_score":4.499809670330265,"endowment":4.499809670330265,"self_citation_contribution":0.6749714505495399,"citation_network_contribution":0.0,"self_endowment_contribution":0.6749714505495399,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":89,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1555997,"name":"T G Boyer","orcid":null,"position":1,"is_corresponding":false},{"id":219571,"name":"A J Berk","orcid":null,"position":2,"is_corresponding":false},{"id":1555996,"name":"Q Zhou","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain.","abstract":"<jats:p>TFIID is a multisubunit protein containing the TATA box-binding polypeptide (TBP) and associated factors (TFIID-TAFs) required for activated transcription by RNA polymerase II. TBPs from different eukaryotes contain a highly conserved carboxy-terminal domain and very divergent amino-terminal domains. Earlier studies proposed that the amino-terminal domains of metazoan TBPs are required for activated transcription. However, we report that a human TFIID complex containing an amino-terminal truncated TBP contains all the major TFIID-TAFs and supports in vitro transcriptional stimulation by different classes of activation domains and from a TATA-less promoter. Protein blotting experiments revealed direct interactions between the conserved domain of TBP and the two largest TAFs. The results suggest a model for the interaction of TFIID-TAFs with TBP.</jats:p>","is_dataset_classified":null,"base_score":4.499809670330265,"endowment":4.499809670330265,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8436290","pmcid":null,"openalex_id":"https://openalex.org/W1990026965","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA 25235","title":null}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2020,"count":1},{"year":2022,"count":1}],"oa_status":"gold","license":null,"oa_locations":[{"url":"http://genesdev.cshlp.org/content/7/2/180.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/7/2/180.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.7.2.180","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.7.2.180","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8436290","host_type":"repository"}],"fields_of_study":["Genomics and Chromatin Dynamics","RNA Research and Splicing","RNA and protein synthesis mechanisms"],"mesh_terms":["Amino Acid Sequence","Binding Sites","DNA-Binding Proteins","HeLa Cells","Humans","Models, Biological","Molecular Sequence Data","Transcription Factors","Transcription, Genetic","Conserved Sequence","TATA-Box Binding Protein","Transcription Factor TFIID","Hela Cells"],"keywords":["TAF1","Transcription factor II D","Transcription factor II A","TATA box","Biology","TATA-Box Binding Protein","TAF4","TATA-binding protein","TAF2","General transcription factor","Cell biology","Genetics","Transcription factor","Molecular biology","DNA-binding protein","Promoter","RNA","RNA polymerase","Gene","Gene expression","Enhancer"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T04:04:31.069573Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}