{"doi":"10.1101/gad.7.12b.2575","title":"p53 domains: identification and characterization of two autonomous DNA-binding regions.","abstract":null,"journal":"Genes & Development","year":1993,"id":643299,"datarank":0.8233406589235032,"base_score":5.488937726156687,"endowment":5.488937726156687,"self_citation_contribution":0.8233406589235032,"citation_network_contribution":0.0,"self_endowment_contribution":0.8233406589235032,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":241,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1673696,"name":"M Reed","orcid":null,"position":1,"is_corresponding":false},{"id":139360,"name":"P Wang","orcid":null,"position":2,"is_corresponding":false},{"id":1673697,"name":"J E Stenger","orcid":null,"position":3,"is_corresponding":false},{"id":1673698,"name":"G Mayr","orcid":null,"position":4,"is_corresponding":false},{"id":1673699,"name":"M E Anderson","orcid":null,"position":5,"is_corresponding":false},{"id":1673700,"name":"J F Schwedes","orcid":null,"position":6,"is_corresponding":false},{"id":1673701,"name":"P Tegtmeyer","orcid":null,"position":7,"is_corresponding":false},{"id":1349806,"name":"Y Wang","orcid":"0009-0002-3907-4544","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"p53 domains: identification and characterization of two autonomous DNA-binding regions.","abstract":"We have investigated the DNA-binding, oligomerization, and trans-activation functions of isolated segments of murine p53. We find that p53 has two autonomous DNA-binding regions. One domain, from amino acid 280 to 390, forms stable tetramers and binds DNA nonspecifically. The biological significance, if any, of this DNA-binding activity is not known. A second domain, from amino acid 80 to 290, does not form stable tetramers under stringent conditions but binds DNA both specifically and nonspecifically. The specific DNA-binding function of p53, therefore, resides in the highly conserved central region of the protein and does not require stable tetramerization. Amino acids 1-290, which include both the specific DNA-binding domain and the amino-terminal acidic region, activate a p53-specific promoter in vivo. This finding strongly argues that the DNA-binding activity of p53 segment 80-290 is physiologically significant. The role of tetramerization in p53 function remains to be determined.","is_dataset_classified":null,"base_score":5.488937726156687,"endowment":5.488937726156687,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8276240","pmcid":null,"openalex_id":"https://openalex.org/W1993831923","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA-28146","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA-18808","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":4},{"year":2014,"count":3},{"year":2015,"count":2},{"year":2016,"count":1},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2022,"count":1},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"gold","license":null,"oa_locations":[{"url":"http://genesdev.cshlp.org/content/7/12b/2575.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/7/12b/2575.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.7.12b.2575","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.7.12b.2575","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8276240","host_type":"repository"}],"fields_of_study":["Cancer-related Molecular Pathways","Molecular Biology Techniques and Applications","RNA modifications and cancer"],"mesh_terms":["Amino Acid Sequence","Animals","Base Sequence","Binding Sites","Chromosome Mapping","DNA","Insecta","Molecular Sequence Data","Phosphorylation","Recombinant Proteins","Transcriptional Activation","Tumor Suppressor Protein p53","Mice"],"keywords":["Biology","Identification (biology)","DNA","Computational biology","Genetics","Molecular biology","Botany"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-08T15:15:13.636486Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}