{"doi":"10.1101/gad.5.8.1430","title":"U1 snRNP can influence 3'-splice site selection as well as 5'-splice site selection.","abstract":"<jats:p>To address the mechanisms that underlie splice site selection and splice site partner assignment, we analyzed the splicing of yeast (Saccharomyces cerevisiae) transcripts containing splice site region duplications. When the 5'-splice site region was duplicated, both sites were utilized to the same extent, indicating little or no influence of proximity on 5'-splice site choice. However, the effect of a 5'-mutant site was greatly enhanced by the presence of an adjacent wild-type site, and this effect was reversed by the restoration of base-pairing with U1 snRNA. 3'-Splice site choice was apparently influenced by proximity, as the site closest to the 5'-splice site was greatly preferred. Studies with strains carrying some U1 snRNA mutations showed an increase in the use of the distal 3'-splice site, indicating a role for U1 snRNP in 3'-splice site selection. The data are compared with those from mammalian splice site choice experiments and suggest mechanisms that influence differential splice site choice as well as exon skipping.</jats:p>","journal":"Genes &amp; Development","year":1991,"id":32040,"datarank":1.9534780365901327,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"self_citation_contribution":0.5244761342199721,"citation_network_contribution":1.4290019023701606,"self_endowment_contribution":0.5244761342199721,"citer_contribution":1.4290019023701606,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":29,"citers_with_citation_signal":27,"citers_with_endowment":27,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":170433,"name":"X L Liao","orcid":null,"position":1,"is_corresponding":false},{"id":170434,"name":"B C Rymond","orcid":null,"position":2,"is_corresponding":false},{"id":170435,"name":"M Rosbash","orcid":null,"position":3,"is_corresponding":false},{"id":170432,"name":"V Goguel","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1831174","pmcid":null,"openalex_id":"https://openalex.org/W2080420502","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM-23549","title":null}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":1,"citation_trend":[{"year":2013,"count":1},{"year":2025,"count":2}],"oa_status":"gold","license":null,"oa_locations":[{"url":"http://genesdev.cshlp.org/content/5/8/1430.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/5/8/1430.full.pdf","host_type":"GOLD"},{"url":"http://genesdev.cshlp.org/content/5/8/1430.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.5.8.1430","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.5.8.1430","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1831174","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","RNA and protein synthesis mechanisms","Genomics and Chromatin Dynamics","Biology","Medicine","Base Sequence","Calorimetry","Chromosome Deletion","Genes, Fungal","Introns","Molecular Sequence Data","Mutagenesis, Site-Directed","Nucleic Acid Conformation","Plasmids","RNA Splicing","RNA, Small Nuclear","Ribonucleoproteins","Ribonucleoproteins, Small Nuclear","Saccharomyces cerevisiae","Transcription, Genetic","beta-Galactosidase"],"mesh_terms":["Base Sequence","beta-Galactosidase","Calorimetry","Chromosome Deletion","Genes, Fungal","Introns","Molecular Sequence Data","Nucleic Acid Conformation","Plasmids","Ribonucleoproteins","RNA Splicing","RNA, Small Nuclear","Saccharomyces cerevisiae","Transcription, Genetic","Mutagenesis, Site-Directed","Ribonucleoproteins, Small Nuclear"],"keywords":["splice","Splice site mutation","snRNP","Biology","RNA splicing","Genetics","Exon","Binding site","Alternative splicing","Gene","RNA"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T10:02:35.368692Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}