{"doi":"10.1101/gad.4.12a.2112","title":"A multisubunit factor, CstF, is required for polyadenylation of mammalian pre-mRNAs.","abstract":null,"journal":"Genes & Development","year":1990,"id":674408,"datarank":0.8239592165010824,"base_score":5.493061443340548,"endowment":5.493061443340548,"self_citation_contribution":0.8239592165010824,"citation_network_contribution":0.0,"self_endowment_contribution":0.8239592165010824,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":242,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":179830,"name":"J L Manley","orcid":null,"position":1,"is_corresponding":false},{"id":1643923,"name":"C C MacDonald","orcid":null,"position":2,"is_corresponding":false},{"id":1643924,"name":"J Wilusz","orcid":null,"position":3,"is_corresponding":false},{"id":169964,"name":"T Shenk","orcid":null,"position":4,"is_corresponding":false},{"id":1762068,"name":"Y Takagaki","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A multisubunit factor, CstF, is required for polyadenylation of mammalian pre-mRNAs.","abstract":"We have purified and characterized a factor required for accurate polyadenylation of mammalian pre-mRNAs in vitro. This factor, called cleavage-stimulation factor (CstF), is composed of three distinct polypeptide subunits of 77, 64, and 50 kD. Using monoclonal antibodies directed against the 64- and 50-kD subunits, we show that CstF is required for efficient cleavage of polyadenylation substrates. Furthermore, CstF present in unfractionated nuclear extracts interacts with pre-mRNAs containing the signal sequence AAUAAA, but not AAGAAA, in such a manner that the 64-kD subunit can be cross-linked to the RNA by UV light. This polypeptide is thus identical to the previously described 64-kD nuclear protein that binds to AAUAAA-containing RNAs. Finally, indirect immunofluorescence of fixed cells indicates that CstF is distributed diffusely throughout the nucleus in a granular pattern distinct from the \"speckled\" pattern displayed by factors involved in pre-mRNA splicing, but similar to that of heterogeneous nuclear ribonucleoproteins. A model is presented in which CstF binds specifically to nascent RNA polymerase II transcripts and, by interacting with other factors, results in a rapid initiation of 3'-end processing of pre-mRNAs.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1980119","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"CA-38965","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM-28983","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":null,"license":null,"oa_locations":[{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.4.12a.2112","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Hela Cells","Humans","Nuclear Proteins","RNA Precursors","RNA, Messenger","Poly A","Antibodies, Monoclonal","Fluorescent Antibody Technique","Precipitin Tests","Electrophoresis, Polyacrylamide Gel","RNA Processing, Post-Transcriptional"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T16:45:57.079042Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}