{"doi":"10.1101/gad.352747.125","title":"A cell type-specific surveillance complex represses cryptic promoters during differentiation in an adult stem cell lineage","abstract":"male germline stem cell lineage, tMAC, a master regulator of spermatocyte differentiation that binds thousands of loci, is required for local opening of chromatin, allowing activation of spermatocyte-specific promoters. Here we show that a cell type-specific surveillance system involving the multiple zinc finger protein Kmg and the pipsqueak domain protein Dany dampens transcriptional output from weak tMAC-dependent promoters and counteracts tMAC binding at thousands of additional cryptic promoters, thus preventing massive expression of aberrant protein-coding transcripts. ChIP-seq showed Kmg enriched at the tMAC-bound promoters that it repressed, consistent with direct action. In contrast, Kmg and Dany did not repress highly expressed tMAC-dependent genes, where they colocalized with their binding partner, the chromatin remodeler Mi-2 (NuRD), along the transcribed regions rather than at the promoter. We discuss a model where Kmg, together with Dany and Mi-2, dampens expression from weak or ectopic promoters while allowing robust transcription from highly expressed Aly-dependent genes.","journal":"Genes & Development","year":2025,"id":571877,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9481,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE301549","GSE301552","GSE301635","GSE301637","GSE301639","GSE301641"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":984222,"name":"Lorenzo Gallicchio","orcid":"0000-0002-5040-0139","position":1,"is_corresponding":false},{"id":551882,"name":"Dan Lu","orcid":"0000-0002-7492-3954","position":2,"is_corresponding":false},{"id":607718,"name":"Jongmin Kim","orcid":"0000-0002-1836-2064","position":3,"is_corresponding":false},{"id":240724,"name":"Julian Perez","orcid":"0000-0003-0022-9967","position":4,"is_corresponding":false},{"id":74074,"name":"Angela M. Detweiler","orcid":"0000-0001-7026-5467","position":5,"is_corresponding":false},{"id":551883,"name":"Chenggang Lu","orcid":"0000-0003-2298-4509","position":6,"is_corresponding":false},{"id":838956,"name":"Benjamin Bolival","orcid":"0000-0001-5734-5112","position":7,"is_corresponding":false},{"id":551884,"name":"Margaret T. Fuller","orcid":"0000-0002-3804-4987","position":8,"is_corresponding":false},{"id":1117054,"name":"Neuza R. Matias","orcid":"0000-0003-1548-037X","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-19T02:57:19.669553Z","pmid":"40769719","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}