{"doi":"10.1101/gad.336024.119","title":"UAP56/DDX39B is a major cotranscriptional RNA–DNA helicase that unwinds harmful R loops genome-wide","abstract":"Nonscheduled R loops represent a major source of DNA damage and replication stress. Cells have different ways to prevent R-loop accumulation. One mechanism relies on the conserved THO complex in association with cotranscriptional RNA processing factors including the RNA-dependent ATPase UAP56/DDX39B and histone modifiers such as the SIN3 deacetylase in humans. We investigated the function of UAP56/DDX39B in R-loop removal. We show that UAP56 depletion causes R-loop accumulation, R-loop-mediated genome instability, and replication fork stalling. We demonstrate an RNA-DNA helicase activity in UAP56 and show that its overexpression suppresses R loops and genome instability induced by depleting five different unrelated factors. UAP56/DDX39B localizes to active chromatin and prevents the accumulation of RNA-DNA hybrids over the entire genome. We propose that, in addition to its RNA processing role, UAP56/DDX39B is a key helicase required to eliminate harmful cotranscriptional RNA structures that otherwise would block transcription and replication.","journal":"Genes & Development","year":2020,"id":118295,"datarank":0.7357912167657645,"base_score":4.90527477843843,"endowment":4.90527477843843,"self_citation_contribution":0.7357912167657645,"citation_network_contribution":0.0,"self_endowment_contribution":0.7357912167657645,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":134,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9559,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":550244,"name":"Aleix Bayona‐Feliú","orcid":"0000-0002-7412-1652","position":1,"is_corresponding":false},{"id":380566,"name":"Xiaoyu Xue","orcid":"0000-0001-9654-6566","position":2,"is_corresponding":false},{"id":550245,"name":"Sónia Barroso","orcid":"0000-0002-0062-2016","position":3,"is_corresponding":false},{"id":550246,"name":"Sergio Muñoz","orcid":"0000-0003-2971-8393","position":4,"is_corresponding":false},{"id":551214,"name":"Víctor Manuel González-Basallote","orcid":null,"position":5,"is_corresponding":false},{"id":278336,"name":"Patrick Sung","orcid":"0000-0003-1396-9040","position":6,"is_corresponding":false},{"id":550247,"name":"Andrés Aguilera","orcid":"0000-0003-4782-1714","position":7,"is_corresponding":false},{"id":551213,"name":"Carmen Pérez‐Calero","orcid":null,"position":0,"is_corresponding":true}],"reference_count":67,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:13:58.531532Z","pmid":"32439635","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}