{"doi":"10.1101/gad.236752.113","title":"Coupling 40S ribosome recruitment to modification of a cap-binding initiation factor by eIF3 subunit e","abstract":"<jats:p>40S ribosomes are loaded onto capped mRNAs via the multisubunit translation initiation factors eIF3 and eIF4F. While eIF4E is the eIF4F cap recognition component, the eIF4G subunit associates with 40S-bound eIF3. How this intricate process is coordinated remains poorly understood. Here, we identify an eIF3 subunit that regulates eIF4F modification and show that eIF3<jats:italic>e</jats:italic> is required for inducible eIF4E phosphorylation. Significantly, recruitment of the eIF4E kinase Mnk1 (MAPK signal-integrating kinase 1) to eIF4F depended on eIF3e, and eIF3e was sufficient to promote Mnk1-binding to eIF4G. This establishes a mechanism by which 40S ribosome loading imparts a phosphorylation mark on the cap-binding eIF4F complex that regulates selective mRNA translation and is synchronized by a specific eIF3 subunit.</jats:p>","journal":"Genes &amp; Development","year":2014,"id":591224,"datarank":1.5709414826521415,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"self_citation_contribution":0.5495342469194471,"citation_network_contribution":1.0214072357326944,"self_endowment_contribution":0.5495342469194471,"citer_contribution":1.0214072357326944,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":30,"citers_with_citation_signal":28,"citers_with_endowment":28,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":584612,"name":"Ian Mohr","orcid":"0000-0002-4921-1998","position":1,"is_corresponding":false},{"id":440246,"name":"Derek Walsh","orcid":"0000-0002-8756-4655","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Coupling 40S ribosome recruitment to modification of a cap-binding initiation factor by eIF3 subunit e","abstract":"<jats:p>40S ribosomes are loaded onto capped mRNAs via the multisubunit translation initiation factors eIF3 and eIF4F. While eIF4E is the eIF4F cap recognition component, the eIF4G subunit associates with 40S-bound eIF3. How this intricate process is coordinated remains poorly understood. Here, we identify an eIF3 subunit that regulates eIF4F modification and show that eIF3<jats:italic>e</jats:italic> is required for inducible eIF4E phosphorylation. Significantly, recruitment of the eIF4E kinase Mnk1 (MAPK signal-integrating kinase 1) to eIF4F depended on eIF3e, and eIF3e was sufficient to promote Mnk1-binding to eIF4G. This establishes a mechanism by which 40S ribosome loading imparts a phosphorylation mark on the cap-binding eIF4F complex that regulates selective mRNA translation and is synchronized by a specific eIF3 subunit.</jats:p>","is_dataset_classified":null,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24736843","pmcid":"PMC4003276","openalex_id":"https://openalex.org/W2059617809","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM056927","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01AI073898","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R21AI105330","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM056927","title":null},{"funder_name":"National Institutes of Health","grant_id":"3R01AI073898-05S1","title":"Virus Host Interactions that Regulate Translation in Cells Infected with HSV-1"},{"funder_name":"National Institutes of Health","grant_id":"5R01GM056927-04","title":"CONTROL OF TRANSLATION IN HERPESVIRUS INFECTED CELLS"},{"funder_name":"National Institutes of Health","grant_id":"5R21AI105330-03","title":"Translational control of gene expression during poxvirus infection"}],"total_grants":7,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2014,"count":4},{"year":2015,"count":7},{"year":2016,"count":2},{"year":2017,"count":4},{"year":2018,"count":5},{"year":2019,"count":2},{"year":2020,"count":4},{"year":2022,"count":3},{"year":2023,"count":2},{"year":2024,"count":3},{"year":2025,"count":2}],"oa_status":"gold","license":"cc-by-nc","oa_locations":[{"url":"http://genesdev.cshlp.org/content/28/8/835.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/28/8/835.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.236752.113","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.236752.113","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24736843","host_type":"repository"},{"url":"http://genesdev.cshlp.org/cgi/content/short/28/8/835","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4003276","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC4003276","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC4003276?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1101/gad.236752.113","host_type":""},{"url":"https://dx.doi.org/10.1101/gad.236752.113","host_type":""}],"fields_of_study":["RNA and protein synthesis mechanisms","RNA modifications and cancer","RNA Research and Splicing","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Chromatography","Humans","Peptide Chain Initiation, Translational","Phosphorylation","Protein Binding","Signal Transduction","Protein Serine-Threonine Kinases","Protein Subunits","Protein Transport","RNA Cap-Binding Proteins","Eukaryotic Initiation Factor-3","Intracellular Signaling Peptides and Proteins","Ribosome Subunits, Small, Eukaryotic"],"keywords":["EIF4E","EIF4G","Biology","Initiation factor","Cell biology","Eukaryotic initiation factor","Eukaryotic Small Ribosomal Subunit","Eukaryotic translation","EIF4A1","Protein subunit","eIF2","Translation (biology)","Genetics","Messenger RNA","Gene","Translation initiation","Eif3","Eif4e Phosphorylation","Mnk1","Ribosome Recruitment","Ribosome Subunits, Small, Eukaryotic","Chromatography","Eukaryotic Initiation Factor-3","Intracellular Signaling Peptides and Proteins","Protein Serine-Threonine Kinases","Research Communication","Protein Subunits","Protein Transport","RNA Cap-Binding Proteins","Humans","Phosphorylation","Peptide Chain Initiation, Translational","Protein Binding","Signal Transduction"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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