{"doi":"10.1101/gad.1989510","title":"Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in <i>Drosophila</i>","abstract":"<jats:p>PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In <jats:italic>Drosophila</jats:italic> ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi–piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.</jats:p>","journal":"Genes &amp; Development","year":2010,"id":593865,"datarank":0.8604858446218789,"base_score":5.736572297479192,"endowment":5.736572297479192,"self_citation_contribution":0.8604858446218789,"citation_network_contribution":0.0,"self_endowment_contribution":0.8604858446218789,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":309,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1519999,"name":"Hirotsugu Ishizu","orcid":null,"position":1,"is_corresponding":false},{"id":1520000,"name":"Miharu Komai","orcid":null,"position":2,"is_corresponding":false},{"id":1520001,"name":"Hazuki Kotani","orcid":null,"position":3,"is_corresponding":false},{"id":1520002,"name":"Yoshinori Kawamura","orcid":null,"position":4,"is_corresponding":false},{"id":1520003,"name":"Kazumichi M. Nishida","orcid":null,"position":5,"is_corresponding":false},{"id":1044337,"name":"Haruhiko Siomi","orcid":"0000-0001-8690-3822","position":6,"is_corresponding":false},{"id":132037,"name":"Mikiko C. Siomi","orcid":null,"position":7,"is_corresponding":false},{"id":259043,"name":"Kuniaki Saito","orcid":"0000-0001-8233-9283","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in <i>Drosophila</i>","abstract":"<jats:p>PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In <jats:italic>Drosophila</jats:italic> ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi–piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.</jats:p>","is_dataset_classified":null,"base_score":5.736572297479192,"endowment":5.736572297479192,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20966047","pmcid":"PMC2975925","openalex_id":"https://openalex.org/W2158912913","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2012,"count":24},{"year":2013,"count":35},{"year":2014,"count":29},{"year":2015,"count":26},{"year":2016,"count":15},{"year":2017,"count":24},{"year":2018,"count":20},{"year":2019,"count":25},{"year":2020,"count":10},{"year":2021,"count":17},{"year":2022,"count":11},{"year":2023,"count":10},{"year":2024,"count":18},{"year":2025,"count":15},{"year":2026,"count":8}],"oa_status":"gold","license":null,"oa_locations":[{"url":"http://genesdev.cshlp.org/content/24/22/2493.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/24/22/2493.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.1989510","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.1989510","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20966047","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2975925","host_type":"repository"}],"fields_of_study":["Chromosomal and Genetic Variations","CRISPR and Genetic Engineering","RNA and protein synthesis mechanisms"],"mesh_terms":["Animals","Cell Line","Cell Nucleus","Cytoplasm","DNA Transposable Elements","Drosophila melanogaster","Endoribonucleases","RNA Helicases","Protein Transport","Drosophila Proteins","RNA, Small Interfering"],"keywords":["Piwi-interacting RNA","RasiRNA","Biology","Transposable element","Drosophila melanogaster","Biogenesis","Genetics","Gene silencing","Cell biology","Genome","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T12:34:12.882322Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}