{"doi":"10.1101/gad.184000","title":"Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye","abstract":"\n The Pax6 transcription factor plays a key role in ocular development of vertebrates and invertebrates. Homozygosity of the\n Pax6\n null mutation in human and mice results in arrest of optic vesicle development and failure to initiate lens formation. This phenotype obscures the understanding of autonomous function of Pax6 in these tissue components and during later developmental stages. We employed the Cre/\n loxP\n approach to inactivate\n Pax6\n specifically in the eye surface ectoderm concomitantly with lens induction. Although lens induction occurred in the mutant, as indicated by\n Sox2\n up-regulation in the surface ectoderm, further development of the lens was arrested. Hence, Pax6 activity was found to be essential in the specified ectoderm for lens placode formation. Furthermore, this mutant model allowed us for the first time to address in vivo the development of a completely normal retina in the absence of early lens structures. Remarkably, several independent, fully differentiated neuroretinas developed in a single optic vesicle in the absence of a lens, demonstrating that the developing lens is not necessary to instruct the differentiation of the neuroretina but is, rather, required for the correct placement of a single retina in the eye.\n ","journal":"Genes & Development","year":2000,"id":24737,"datarank":11.884212993191221,"base_score":6.361302477572996,"endowment":6.361302477572996,"self_citation_contribution":0.9541953716359495,"citation_network_contribution":10.930017621555272,"self_endowment_contribution":0.9541953716359495,"citer_contribution":10.930017621555272,"corpus_percentile":null,"corpus_rank":null,"citation_count":578,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":147523,"name":"Till Marquardt","orcid":null,"position":1,"is_corresponding":false},{"id":147524,"name":"Xunlei Zhou","orcid":null,"position":2,"is_corresponding":false},{"id":147525,"name":"Peter Gruss","orcid":null,"position":3,"is_corresponding":false},{"id":147522,"name":"Ruth Ashery-Padan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":6.361302477572996,"endowment":6.361302477572996,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11069887","pmcid":"PMC317031","openalex_id":"https://openalex.org/W2134321528","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":61,"citation_trend":[{"year":2012,"count":23},{"year":2013,"count":23},{"year":2014,"count":25},{"year":2015,"count":24},{"year":2016,"count":29},{"year":2017,"count":13},{"year":2018,"count":21},{"year":2019,"count":23},{"year":2020,"count":13},{"year":2021,"count":13},{"year":2022,"count":15},{"year":2023,"count":18},{"year":2024,"count":21},{"year":2025,"count":14},{"year":2026,"count":3}],"oa_status":"gold","license":"other-oa","oa_locations":[{"url":"http://genesdev.cshlp.org/content/14/21/2701.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/14/21/2701.full.pdf","host_type":"GOLD"},{"url":"http://genesdev.cshlp.org/content/14/21/2701.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.184000","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.184000","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11069887","host_type":"repository"},{"url":"http://edoc.mpg.de/222591","host_type":"repository"},{"url":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-2018-224074%22","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/317031","host_type":"repository"},{"url":"http://hdl.handle.net/11858/00-001M-0000-0012-F7D0-5","host_type":"repository"}],"fields_of_study":["Retinal Development and Disorders","Connexins and lens biology","Developmental Biology and Gene Regulation","Biology","Medicine","Animals","Animals, Newborn","DNA-Binding Proteins","Ectoderm","Eye Abnormalities","Eye Proteins","Feedback","Gene Deletion","Gene Expression Regulation, Developmental","HMGB Proteins","Homeodomain Proteins","Humans","Lens, Crystalline","Mice","Mice, Knockout","Mice, Mutant Strains","Morphogenesis","Nuclear Proteins","PAX6 Transcription Factor","Paired Box Transcription Factors","Repressor Proteins","Retina","SOXB1 Transcription Factors","Transcription Factors"],"mesh_terms":["PAX6 Transcription Factor","Animals","Animals, Newborn","DNA-Binding Proteins","Ectoderm","Eye Abnormalities","Eye Proteins","Feedback","Humans","Lens, Crystalline","Mice, Mutant Strains","Morphogenesis","Nuclear Proteins","Repressor Proteins","Retina","Transcription Factors","Gene Deletion","Mice, Knockout","Homeodomain Proteins","Gene Expression Regulation, Developmental","HMGB Proteins","Mice","Paired Box Transcription Factors","SOXB1 Transcription Factors"],"keywords":["PAX6","Optic vesicle","Eye development","Biology","Ectoderm","Lens (geology)","Cell biology","Retina","Optic cup (embryology)","Mutant","Transcription factor","Genetics","Embryogenesis","Embryo","Neuroscience","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-07T23:07:28.870431Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}