{"doi":"10.1101/gad.1352305","title":"Polycomb mediates Myc autorepression and its transcriptional control of many loci in <i>Drosophila</i>","abstract":"<jats:p>Aberrant accumulation of the Myc oncoprotein propels proliferation and induces carcinogenesis. In normal cells, however, an abundance of Myc protein represses transcription at the c-<jats:italic>myc</jats:italic> locus. Cancer cells often lose this autorepression. We examined the control of <jats:italic>myc</jats:italic> in <jats:italic>Drosophila</jats:italic> and show here that the <jats:italic>Drosophila</jats:italic> ortholog, <jats:italic>dmyc,</jats:italic> also undergoes autorepression. We find that the developmental repressor Polycomb (Pc) is required for <jats:italic>dmyc</jats:italic> autorepression, and that this Pc-dMyc-mediated repression spreads across an 875-kb region encompassing the <jats:italic>dmyc</jats:italic> gene. To further investigate the relationship between Myc and Polycomb, we used microarrays to identify genes regulated by each, and identify a striking relationship between the two: A large set of dMyc activation targets is normally repressed by Pc, and 73% of dMyc repression targets require Pc for this repression. Chromatin immunoprecipitation confirmed that many dMyc-Pc-repressed loci have an epigenetic mark recognized by Pc. Our results suggest a novel relationship between Myc and Polycomb, wherein Myc enhances Polycomb repression in order to repress targets, and Myc suppresses Polycomb repression in order to activate targets.</jats:p>","journal":"Genes &amp; Development","year":2005,"id":623981,"datarank":2.0316051243965774,"base_score":3.8501476017100584,"endowment":3.8501476017100584,"self_citation_contribution":0.5775221402565088,"citation_network_contribution":1.4540829841400684,"self_endowment_contribution":0.5775221402565088,"citer_contribution":1.4540829841400684,"corpus_percentile":null,"corpus_rank":null,"citation_count":46,"citer_count":40,"citers_with_citation_signal":37,"citers_with_endowment":37,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":347420,"name":"Eric Wieschaus","orcid":"0000-0002-0727-3349","position":1,"is_corresponding":false},{"id":771911,"name":"Michael D. Cole","orcid":"0000-0002-3421-5451","position":2,"is_corresponding":false},{"id":1612912,"name":"Julie M. Goodliffe","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Polycomb mediates Myc autorepression and its transcriptional control of many loci in <i>Drosophila</i>","abstract":"<jats:p>Aberrant accumulation of the Myc oncoprotein propels proliferation and induces carcinogenesis. In normal cells, however, an abundance of Myc protein represses transcription at the c-<jats:italic>myc</jats:italic> locus. Cancer cells often lose this autorepression. We examined the control of <jats:italic>myc</jats:italic> in <jats:italic>Drosophila</jats:italic> and show here that the <jats:italic>Drosophila</jats:italic> ortholog, <jats:italic>dmyc,</jats:italic> also undergoes autorepression. We find that the developmental repressor Polycomb (Pc) is required for <jats:italic>dmyc</jats:italic> autorepression, and that this Pc-dMyc-mediated repression spreads across an 875-kb region encompassing the <jats:italic>dmyc</jats:italic> gene. To further investigate the relationship between Myc and Polycomb, we used microarrays to identify genes regulated by each, and identify a striking relationship between the two: A large set of dMyc activation targets is normally repressed by Pc, and 73% of dMyc repression targets require Pc for this repression. Chromatin immunoprecipitation confirmed that many dMyc-Pc-repressed loci have an epigenetic mark recognized by Pc. Our results suggest a novel relationship between Myc and Polycomb, wherein Myc enhances Polycomb repression in order to repress targets, and Myc suppresses Polycomb repression in order to activate targets.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16357214","pmcid":"PMC1315398","openalex_id":null,"authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"R01 CA080320","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA055248","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":null,"oa_locations":[{"url":"http://genesdev.cshlp.org/content/19/24/2941.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.1352305","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Chromatin","Animals","Drosophila","DNA-Binding Proteins","Drosophila Proteins","Transcription Factors","Oligonucleotide Array Sequence Analysis","Chromatin Immunoprecipitation","Gene Expression Profiling","Down-Regulation","Gene Expression Regulation, Developmental","Quantitative Trait Loci","Polycomb Repressive Complex 1"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T02:12:53.511900Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}