{"doi":"10.1101/gad.13.14.1780","title":"Antagonism between Goalpha  and Gqalpha  in Caenorhabditis elegans: the RGS protein EAT-16 is necessary for Goalpha  signaling and regulates Gqalpha  activity","abstract":null,"journal":"Genes & Development","year":1999,"id":591903,"datarank":8.673769606530428,"base_score":5.2574953720277815,"endowment":5.2574953720277815,"self_citation_contribution":0.7886243058041673,"citation_network_contribution":7.885145300726261,"self_endowment_contribution":0.7886243058041673,"citer_contribution":7.885145300726261,"corpus_percentile":null,"corpus_rank":null,"citation_count":191,"citer_count":168,"citers_with_citation_signal":148,"citers_with_endowment":148,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1514493,"name":"W. J. Chen","orcid":null,"position":1,"is_corresponding":false},{"id":1514494,"name":"G. Patikoglou","orcid":null,"position":2,"is_corresponding":false},{"id":1514495,"name":"M. R. Koelle","orcid":null,"position":3,"is_corresponding":false},{"id":1514496,"name":"P. W. Sternberg","orcid":null,"position":4,"is_corresponding":false},{"id":1514492,"name":"Y. M. Hajdu-Cronin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Antagonism between Goalpha  and Gqalpha  in Caenorhabditis elegans: the RGS protein EAT-16 is necessary for Goalpha  signaling and regulates Gqalpha  activity","abstract":"To elucidate the cellular role of the heterotrimeric G protein G(o), we have taken a molecular genetic approach in Caenorhabditis elegans. We screened for suppressors of activated GOA-1 (G(o)alpha) that do not simply decrease its expression and found mutations in only two genes, sag-1 and eat-16. Animals defective in either gene display a hyperactive phenotype similar to that of goa-1 loss-of-function mutants. Double-mutant analysis indicates that both sag-1 and eat-16 act downstream of, or parallel to, G(o)alpha and negatively regulate EGL-30 (G(q)alpha) signaling. eat-16 encodes a regulator of G protein signaling (RGS) most similar to the mammalian RGS7 and RGS9 proteins and can inhibit endogenous mammalian G(q)/G(11) in COS-7 cells. Animals defective in both sag-1 and eat-16 are inviable, but reducing function in egl-30 restores viability, indicating that the lethality of the eat-16; sag-1 double mutant is due to excessive G(q)alpha activity. Analysis of these mutations indicates that the G(o) and G(q) pathways function antagonistically in C. elegans, and that G(o)alpha negatively regulates the G(q) pathway, possibly via EAT-16 or SAG-1. We propose that a major cellular role of G(o) is to antagonize signaling by G(q).","is_dataset_classified":null,"base_score":5.2574953720277815,"endowment":5.2574953720277815,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10421631","pmcid":"PMC316886","openalex_id":"https://openalex.org/W2187773005","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":7},{"year":2014,"count":8},{"year":2015,"count":5},{"year":2016,"count":6},{"year":2017,"count":5},{"year":2020,"count":3},{"year":2021,"count":6},{"year":2022,"count":4},{"year":2023,"count":3},{"year":2024,"count":5},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"gold","license":null,"oa_locations":[{"url":"http://genesdev.cshlp.org/content/13/14/1780.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/13/14/1780.full.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.13.14.1780","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10421631","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/316886","host_type":"repository"}],"fields_of_study":["Genetics, Aging, and Longevity in Model Organisms","Neurobiology and Insect Physiology Research","Hormonal Regulation and Hypertension"],"mesh_terms":["Amino Acid Sequence","Animals","Base Sequence","Gene Expression Regulation","Molecular Sequence Data","Mutation","Signal Transduction","Helminth Proteins","Genes, Suppressor","Caenorhabditis elegans","Sequence Homology, Amino Acid","DNA Primers","GTP-Binding Proteins","COS Cells","GTP-Binding Protein Regulators","Caenorhabditis elegans Proteins"],"keywords":["Heterotrimeric G protein","Caenorhabditis elegans","Biology","Gq alpha subunit","Mutant","G protein","Cell biology","Gs alpha subunit","Signal transduction","Phenotype","GTPase-activating protein","Gene","Genetic screen","G alpha subunit","Mutation","Genetics","Protein subunit"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-26T11:17:30.297737Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}