{"doi":"10.1101/gad.10.16.2025","title":"A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae.","abstract":"<jats:p>With the use of an intrachromosomal inverted-repeat as a recombination reporter we have previously shown that mitotic recombination is dependent on the RAD52 gene. However, recombination was found to be reduced only 4-fold by mutation of RAD51, which encodes a homolog of bacterial RecA proteins. A rad51, which strain containing the recombination reporter was mutagenized to identify components of the RAD51-independent pathway. One mutation identified, rad59, reduced recombination 1200-fold in the presence of a rad51 mutation, but only 4- to 5-fold in a wild-type background. Thus the rad51 and rad59 mutations reduce recombination synergistically. The rad59 mutation reduced both spontaneous and double-strand-break-induced recombination between inverted repeats. However, the rate of interchromosomal recombination was increased in a rad59 homozygous diploid. These observations suggest that RAD59 functions specifically in intrachromosomal recombination. The rad59 mutant strain was sensitive to ionizing radiation, and this phenotype was used to clone the RAD59 gene by complementation. The gene encodes a protein of 238 amino acids with significant homology to members of the Rad52 family. Overexpression of RAD52 was found to suppress the DNA repair and recombination defects conferred by the rad59 mutation, suggesting that these proteins have overlapping roles or function as a complex.</jats:p>","journal":"Genes &amp; Development","year":1996,"id":16482,"datarank":11.34108487065389,"base_score":5.4510384535657,"endowment":5.4510384535657,"self_citation_contribution":0.8176557680348552,"citation_network_contribution":10.523429102619035,"self_endowment_contribution":0.8176557680348552,"citer_contribution":10.523429102619035,"corpus_percentile":null,"corpus_rank":null,"citation_count":232,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":121498,"name":"L S Symington","orcid":null,"position":1,"is_corresponding":false},{"id":121497,"name":"Y Bai","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.4510384535657,"endowment":5.4510384535657,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8769646","pmcid":null,"openalex_id":"https://openalex.org/W2102684624","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM041784","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM41784","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":26,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":5},{"year":2014,"count":10},{"year":2015,"count":1},{"year":2016,"count":6},{"year":2017,"count":3},{"year":2018,"count":5},{"year":2019,"count":3},{"year":2020,"count":9},{"year":2021,"count":5},{"year":2022,"count":5},{"year":2023,"count":2},{"year":2025,"count":2}],"oa_status":"gold","license":null,"oa_locations":[{"url":"http://genesdev.cshlp.org/content/10/16/2025.full.pdf","host_type":"journal"},{"url":"http://genesdev.cshlp.org/content/10/16/2025.full.pdf","host_type":"GOLD"},{"url":"http://genesdev.cshlp.org/content/10/16/2025.full.pdf","host_type":"publisher"},{"url":"https://syndication.highwire.org/content/doi/10.1101/gad.10.16.2025","host_type":"publisher"},{"url":"https://doi.org/10.1101/gad.10.16.2025","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8769646","host_type":"repository"},{"url":"http://genesdev.cshlp.org/cgi/content/short/10/16/2025","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","CRISPR and Genetic Engineering","Fungal and yeast genetics research","Biology","Medicine","Amino Acid Sequence","Base Sequence","Cloning, Molecular","DNA Primers","DNA Repair","DNA-Binding Proteins","Fungal Proteins","Gamma Rays","Gene Conversion","Gene Expression Regulation, Fungal","Genes, Fungal","Genetic Complementation Test","Mitosis","Molecular Sequence Data","Rad51 Recombinase","Rad52 DNA Repair and Recombination Protein","Recombination, Genetic","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","Sequence Alignment","Sequence Homology, Amino Acid"],"mesh_terms":["Amino Acid Sequence","Base Sequence","Cloning, Molecular","DNA Repair","DNA-Binding Proteins","Fungal Proteins","Gamma Rays","Gene Conversion","Genes, Fungal","Genetic Complementation Test","Mitosis","Molecular Sequence Data","Recombination, Genetic","Saccharomyces cerevisiae","Gene Expression Regulation, Fungal","Sequence Alignment","Sequence Homology, Amino Acid","DNA Primers","Saccharomyces cerevisiae Proteins","Rad51 Recombinase","Rad52 DNA Repair and Recombination Protein"],"keywords":["RAD52","RAD51","Mitotic crossover","Biology","FLP-FRT recombination","Homologous recombination","Ectopic recombination","Genetics","Non-homologous end joining","Genetic recombination","Molecular biology","Mutant","Saccharomyces cerevisiae","Mutation","Recombination","DNA repair","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-02T09:24:01.390099Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}