{"doi":"10.1101/812503","title":"<i>Tetrahymena</i>\n                  Poc5 is a transient basal body component that is important for basal body maturation","abstract":"<jats:title>ABSTRACT</jats:title>\n                <jats:p>\n                  Basal bodies (BBs) are microtubule-based organelles that template and stabilize cilia at the cell surface. Centrins ubiquitously associate with BBs and function in BB assembly, maturation, and stability. Human POC5 (hPOC5) is a highly conserved centrin-binding protein that binds centrins through Sfi1p-like repeats and is required for building full-length, mature centrioles. Here, we use the BB-rich cytoskeleton of\n                  <jats:italic>Tetrahymena thermophila</jats:italic>\n                  to characterize Poc5 BB functions.\n                  <jats:italic>Tetrahymena</jats:italic>\n                  Poc5 (TtPoc5) uniquely incorporates into assembling BBs and is then removed from mature BBs prior to ciliogenesis. Complete genomic knockout of\n                  <jats:italic>TtPOC5</jats:italic>\n                  leads to a significantly increased production of BBs yet a markedly reduced ciliary density, both of which are rescued by reintroduction of TtPoc5. A second\n                  <jats:italic>Tetrahymena POC5</jats:italic>\n                  -like gene,\n                  <jats:italic>SFR1</jats:italic>\n                  , is similarly implicated in modulating BB production. When\n                  <jats:italic>TtPOC5</jats:italic>\n                  and\n                  <jats:italic>SFR1</jats:italic>\n                  are co-deleted, cell viability is compromised, and levels of BB overproduction are exacerbated. Overproduced BBs display defective transition zone formation and a diminished capacity for ciliogenesis. This study uncovers a requirement for Poc5 in building mature BBs, providing a possible functional link between\n                  <jats:italic>hPOC5</jats:italic>\n                  mutations and impaired cilia.\n                </jats:p>\n                <jats:sec>\n                  <jats:title>SUMMARY STATEMENT</jats:title>\n                  <jats:p>\n                    Loss of\n                    <jats:italic>Tetrahymena thermophila</jats:italic>\n                    Poc5 reveals an important role for this centrin-binding protein in basal body maturation, which also impacts basal body production and ciliogenesis.\n                  </jats:p>\n                </jats:sec>","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":null,"id":17762,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":125932,"name":"Brian A. Bayless","orcid":null,"position":1,"is_corresponding":false},{"id":125933,"name":"Alexander J. Stemm-Wolf","orcid":null,"position":2,"is_corresponding":false},{"id":125934,"name":"Eileen T. O’Toole","orcid":null,"position":3,"is_corresponding":false},{"id":125935,"name":"Courtney Ozzello","orcid":null,"position":4,"is_corresponding":false},{"id":125936,"name":"Marina Nguyen","orcid":null,"position":5,"is_corresponding":false},{"id":125675,"name":"Mark Winey","orcid":"0000-0002-9028-9033","position":6,"is_corresponding":false},{"id":125931,"name":"Westley Heydeck","orcid":"0000-0002-8706-9865","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32350068","pmcid":null,"openalex_id":"https://openalex.org/W2981030967","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"2R01GM074746-05A1","title":"Tetrahymena Basal Body Duplication"},{"funder_name":"National Institutes of Health","grant_id":"5R01GM127571-03","title":"EFHC gene function in ciliary axomenes."}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":1,"citation_trend":[],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2019/10/21/812503.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2019/10/21/812503.full.pdf","host_type":"BRONZE"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2019/10/21/812503.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/812503","host_type":"publisher"},{"url":"https://doi.org/10.1101/812503","host_type":"repository"},{"url":"https://jcs.biologists.org/content/joces/133/11/jcs240838.full.pdf","host_type":""},{"url":"https://dx.doi.org/10.1101/812503","host_type":""},{"url":"http://dx.doi.org/10.1101/812503","host_type":""}],"fields_of_study":["Genetic and Kidney Cyst Diseases","Protist diversity and phylogeny","Microtubule and mitosis dynamics","Biology","Medicine","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Ciliogenesis","Tetrahymena","Basal body","Cilium","Biology","Cell biology","Centriole","Organelle","Microtubule","Ciliopathy","Genetics","Gene","Phenotype","Flagellum"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-02T22:26:41.347761Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}