{"doi":"10.1101/616227","title":"Mapping cell migrations and fates from a gastruloid model to the human primitive streak","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>Although fate maps of early gastrula embryos exist for nearly all model organisms, a fate map of the gastrulating human embryo remains elusive. Here we use human gastruloids to piece together part of a rudimentary fate map of the human primitive streak (PS). This is possible because stimulation with differing levels of BMP, WNT, and NODAL leads to self-organization of gastruloids into large and homogenous different subpopulations of endoderm and mesoderm, and comparative parallel analysis of these gastruloids, together with the fate map of the mouse embryo, allows the organization of these subpopulations along an anterior-posterior axis. We also developed a novel cell tracking technique that allowed the detection of robust fate-dependent cell migrations in our gastruloids comparable to those found in the mouse embryo. Taken together, our gastruloid derived fate map and recording of cell migrations provides a first coarse view of the embryonic human PS.</jats:p>","journal":null,"year":null,"id":590227,"datarank":0.8154110767745708,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.3660512357414722,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.3660512357414722,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":17,"citers_with_citation_signal":14,"citers_with_endowment":14,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":438172,"name":"Eric D. Siggia","orcid":"0000-0001-7482-1854","position":1,"is_corresponding":false},{"id":532141,"name":"Ali H. Brivanlou","orcid":"0000-0002-1761-280X","position":2,"is_corresponding":false},{"id":1510179,"name":"I. Martyn","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Mapping cell migrations and fates from a gastruloid model to the human primitive streak","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>Although fate maps of early gastrula embryos exist for nearly all model organisms, a fate map of the gastrulating human embryo remains elusive. Here we use human gastruloids to piece together part of a rudimentary fate map of the human primitive streak (PS). This is possible because stimulation with differing levels of BMP, WNT, and NODAL leads to self-organization of gastruloids into large and homogenous different subpopulations of endoderm and mesoderm, and comparative parallel analysis of these gastruloids, together with the fate map of the mouse embryo, allows the organization of these subpopulations along an anterior-posterior axis. We also developed a novel cell tracking technique that allowed the detection of robust fate-dependent cell migrations in our gastruloids comparable to those found in the mouse embryo. Taken together, our gastruloid derived fate map and recording of cell migrations provides a first coarse view of the embryonic human PS.</jats:p>","is_dataset_classified":null,"base_score":2.995732273553991,"endowment":2.995732273553991,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20725694","pmcid":null,"openalex_id":"https://openalex.org/W2941332545","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"1R01HD080699-01","title":"Dissecting embryonic axis formation using micropatterned hESC colony architecture"},{"funder_name":"National Institutes of Health","grant_id":"5R01GM101653-07","title":"Combining dynamics of ligand presentation with dynamics of hESC response in colonies with defined architecture"}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2020,"count":3},{"year":2021,"count":5},{"year":2022,"count":2},{"year":2023,"count":2},{"year":2024,"count":1},{"year":2025,"count":6}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2019/04/23/616227.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2019/04/23/616227.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/616227","host_type":"publisher"},{"url":"https://doi.org/10.1101/616227","host_type":"repository"},{"url":"https://doi.org/10.1242/dev.179564","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/31427289","host_type":""},{"url":"https://dx.doi.org/10.1101/616227","host_type":""},{"url":"https://dx.doi.org/10.1242/dev.179564","host_type":""},{"url":"http://dx.doi.org/10.1101/616227","host_type":""}],"fields_of_study":["Congenital heart defects research","Pluripotent Stem Cells Research","Developmental Biology and Gene Regulation","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Primitive streak","Gastrulation","Fate mapping","Mesoderm","Cell fate determination","Endoderm","Embryo","Biology","NODAL","Embryonic stem cell","Cell biology","Nodal signaling","Evolutionary biology","Embryogenesis","Anatomy","Genetics","Gene","Human Embryonic Stem Cells","Cell Differentiation","Gastrula","Fibroblasts","Embryo, Mammalian","Models, Biological","Mice","Cell Movement","Cell Tracking","Animals","Humans","Cells, Cultured","Germ Layers"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-24T14:42:17.579981Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}