{"doi":"10.1101/562512","title":"CD29 marks superior cytotoxic human T cells","abstract":"<jats:title>ABSTRACT</jats:title>\n                <jats:p>\n                  Cytotoxic CD8\n                  <jats:sup>+</jats:sup>\n                  T cells can effectively kill target cells by producing cytokines, chemokines and granzymes. Expression of these effector molecules is however highly divergent, and tools that identify and pre-select potent killer cells are lacking. Human CD8\n                  <jats:sup>+</jats:sup>\n                  T cells can be divided into IFN-γ and IL-2 producing cells. Unbiased RNA-sequencing and proteomics analysis on cytokine-producing fixed cells revealed that IL-2\n                  <jats:sup>+</jats:sup>\n                  T cells produce helper cytokines, and that IFN-γ\n                  <jats:sup>+</jats:sup>\n                  T cells produce cytotoxic molecules. IFN-γ\n                  <jats:sup>+</jats:sup>\n                  T cells could be identified with the surface marker CD29 already prior to stimulation. CD29 also marked T cells with cytotoxic gene expression from different tissues in single-cell RNA-sequencing data. Notably, the cytotoxic features of CD29\n                  <jats:sup>+</jats:sup>\n                  T cells were maintained during cell culture, suggesting a stable phenotype. Pre-selecting CD29-expressing MART1 TCR-engineered T cells potentiated the killing of target cells. We therefore propose that selecting for CD29\n                  <jats:sup>+</jats:sup>\n                  T cells could boost the anti-tumoral activity of T cell therapeutics.\n                </jats:p>","journal":null,"year":null,"id":593009,"datarank":0.43530131591969307,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.14341479356139603,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.14341479356139603,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":5,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1517610,"name":"Aurélie Guislain","orcid":null,"position":1,"is_corresponding":false},{"id":1517611,"name":"Floris P.J. van Alphen","orcid":null,"position":2,"is_corresponding":false},{"id":1517612,"name":"Raquel Gomez-Eerland","orcid":null,"position":3,"is_corresponding":false},{"id":1517614,"name":"Ton N. M. Schumacher","orcid":null,"position":4,"is_corresponding":false},{"id":789832,"name":"Maartje van den Biggelaar","orcid":"0000-0001-6970-5496","position":5,"is_corresponding":false},{"id":1517617,"name":"Monika C. Wolkers","orcid":null,"position":6,"is_corresponding":false},{"id":1517609,"name":"Benoît P. Nicolet","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"CD29 marks superior cytotoxic human T cells","abstract":"<jats:title>ABSTRACT</jats:title>\n                <jats:p>\n                  Cytotoxic CD8\n                  <jats:sup>+</jats:sup>\n                  T cells can effectively kill target cells by producing cytokines, chemokines and granzymes. Expression of these effector molecules is however highly divergent, and tools that identify and pre-select potent killer cells are lacking. Human CD8\n                  <jats:sup>+</jats:sup>\n                  T cells can be divided into IFN-γ and IL-2 producing cells. Unbiased RNA-sequencing and proteomics analysis on cytokine-producing fixed cells revealed that IL-2\n                  <jats:sup>+</jats:sup>\n                  T cells produce helper cytokines, and that IFN-γ\n                  <jats:sup>+</jats:sup>\n                  T cells produce cytotoxic molecules. IFN-γ\n                  <jats:sup>+</jats:sup>\n                  T cells could be identified with the surface marker CD29 already prior to stimulation. CD29 also marked T cells with cytotoxic gene expression from different tissues in single-cell RNA-sequencing data. Notably, the cytotoxic features of CD29\n                  <jats:sup>+</jats:sup>\n                  T cells were maintained during cell culture, suggesting a stable phenotype. Pre-selecting CD29-expressing MART1 TCR-engineered T cells potentiated the killing of target cells. We therefore propose that selecting for CD29\n                  <jats:sup>+</jats:sup>\n                  T cells could boost the anti-tumoral activity of T cell therapeutics.\n                </jats:p>","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26657633","pmcid":null,"openalex_id":"https://openalex.org/W2915343159","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2019,"count":1},{"year":2020,"count":3},{"year":2024,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2019/08/09/562512.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2019/08/09/562512.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/562512","host_type":"publisher"},{"url":"https://doi.org/10.1101/562512","host_type":"repository"}],"fields_of_study":["Immune Cell Function and Interaction","CAR-T cell therapy research","T-cell and B-cell Immunology"],"mesh_terms":[],"keywords":["Cytotoxic T cell","Interleukin 21","Cell biology","Antigen-presenting cell","Biology","Natural killer T cell","Granzyme","ZAP70","T cell","CD8","Interleukin 12","IL-2 receptor","Molecular biology","Immunology","Perforin","Immune system","In vitro","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-26T16:38:35.440675Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}