{"doi":"10.1101/358242","title":"MEIOB and SPATA22 resemble RPA subunits and interact with the RPA complex to promote meiotic recombination","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Homologous recombination is a conserved DNA repair process mandatory for chromosome segregation during meiosis. RPA, a ubiquitous complex essential to recombination, is thought to play a similar role during mitotic and meiotic recombination. MEIOB, a meiosis-specific factor with unknown molecular function, ressembles a RPA subunit. Here we use\n                  <jats:italic>in vivo</jats:italic>\n                  approaches to show that in mouse spermatocytes, DMC1 and RAD51 appear to be normally loaded in the absence of MEIOB but are prematurely lost from unrepaired recombination sites. This loss correlates with an accumulation of the BLM helicase on meiotic chromosomes. We also show that MEIOB alters the immunodetection of RPA subunits at meiotic recombination sites. Using electron microscopy and purified proteins, we demonstrate that the MEIOB-SPATA22 complex associates with and modifies the conformation of RPA-coated ssDNA. Finally, we identify structural homology between MEIOB, SPATA22 and RPA subunits, and show that MEIOB and SPATA22 interact through C-terminal OB-fold containing domains (OBCDs) like RPA subunits. Moreover, MEIOB and SPATA22 cooperate to interact with RPA through their OBCDs. Our results suggest that MEIOB, SPATA22 and RPA work together to ensure proper processing of meiotic recombination intermediates.\n                </jats:p>","journal":null,"year":null,"id":686035,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":202169,"name":"Pauline Dupaigne","orcid":null,"position":1,"is_corresponding":false},{"id":1792366,"name":"Clotilde Duquenne","orcid":null,"position":2,"is_corresponding":false},{"id":1792367,"name":"Xavier Veaute","orcid":null,"position":3,"is_corresponding":false},{"id":389545,"name":"Cynthia Petrillo","orcid":null,"position":4,"is_corresponding":false},{"id":653874,"name":"Carole Saintomé","orcid":"0000-0002-7609-6913","position":5,"is_corresponding":false},{"id":647576,"name":"Orestis Faklaris","orcid":"0000-0001-5965-5405","position":6,"is_corresponding":false},{"id":1792369,"name":"Didier Busso","orcid":null,"position":7,"is_corresponding":false},{"id":1792370,"name":"Raphaël Guerois","orcid":null,"position":8,"is_corresponding":false},{"id":254578,"name":"Scott Keeney","orcid":"0000-0002-1283-6417","position":9,"is_corresponding":false},{"id":388514,"name":"Devanshi Jain","orcid":"0000-0002-5027-0549","position":10,"is_corresponding":false},{"id":202175,"name":"Emmanuelle Martini","orcid":null,"position":11,"is_corresponding":false},{"id":202174,"name":"Gabriel Livera","orcid":null,"position":12,"is_corresponding":false},{"id":389546,"name":"Jonathan Ribeiro","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"MEIOB and SPATA22 resemble RPA subunits and interact with the RPA complex to promote meiotic recombination","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Homologous recombination is a conserved DNA repair process mandatory for chromosome segregation during meiosis. RPA, a ubiquitous complex essential to recombination, is thought to play a similar role during mitotic and meiotic recombination. MEIOB, a meiosis-specific factor with unknown molecular function, ressembles a RPA subunit. Here we use\n                  <jats:italic>in vivo</jats:italic>\n                  approaches to show that in mouse spermatocytes, DMC1 and RAD51 appear to be normally loaded in the absence of MEIOB but are prematurely lost from unrepaired recombination sites. This loss correlates with an accumulation of the BLM helicase on meiotic chromosomes. We also show that MEIOB alters the immunodetection of RPA subunits at meiotic recombination sites. Using electron microscopy and purified proteins, we demonstrate that the MEIOB-SPATA22 complex associates with and modifies the conformation of RPA-coated ssDNA. Finally, we identify structural homology between MEIOB, SPATA22 and RPA subunits, and show that MEIOB and SPATA22 interact through C-terminal OB-fold containing domains (OBCDs) like RPA subunits. Moreover, MEIOB and SPATA22 cooperate to interact with RPA through their OBCDs. Our results suggest that MEIOB, SPATA22 and RPA work together to ensure proper processing of meiotic recombination intermediates.\n                </jats:p>","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26207759","pmcid":null,"openalex_id":"https://openalex.org/W2810222002","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2019,"count":2},{"year":2020,"count":2},{"year":2025,"count":1}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2018/06/28/358242.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2018/06/28/358242.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/358242","host_type":"publisher"},{"url":"https://doi.org/10.1101/358242","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","CRISPR and Genetic Engineering","Mitochondrial Function and Pathology"],"mesh_terms":[],"keywords":["Homologous recombination","RAD51","Meiosis","Homologous chromosome","Recombination","Biology","Helicase","Genetic recombination","Replication protein A","Cell biology","Chromosome segregation","Genetics","Chromosomal crossover","DNA","Chromosome","DNA-binding protein","Gene","Transcription factor"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T18:23:45.537241Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}