{"doi":"10.1101/329631","title":"Luciferase of the Japanese syllid polychaete\n                  <i>Odontosyllis umdecimdonta</i>","abstract":"<jats:label>1</jats:label>\n                <jats:title>Abstract</jats:title>\n                <jats:p>\n                  <jats:italic>Odontosyllis undecimdonta</jats:italic>\n                  is a marine syllid polychaete that produces bright internal and exuded bioluminescence. Despite over fifty years of biochemical investigation into\n                  <jats:italic>Odontosyllis</jats:italic>\n                  bioluminescence, the light-emitting small molecule substrate and catalyzing luciferase protein have remained a mystery. Here we describe the discovery of a bioluminescent protein fraction from\n                  <jats:italic>O. undecimdonta,</jats:italic>\n                  the identification of the luciferase using peptide and RNA sequencing, and the\n                  <jats:italic>in vitro</jats:italic>\n                  reconstruction of the bioluminescence reaction using highly purified\n                  <jats:italic>O. undecimdonta</jats:italic>\n                  luciferin and recombinant luciferase. Lastly, we found no identifiably homologous proteins in publicly available datasets. This suggests that the syllid polychaetes contain an evolutionarily unique luciferase among all characterized luminous taxa.\n                </jats:p>\n                <jats:sec>\n                  <jats:label>3</jats:label>\n                  <jats:title>Highlights</jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>\n                          The polychaete\n                          <jats:italic>O. undecimdonta</jats:italic>\n                          uses a luciferin-luciferase bioluminescence system\n                        </jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          <jats:italic>O. undecimdonta</jats:italic>\n                          bioluminescence does not require additional cofactors\n                        </jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>The luciferase of the Japanese fireworm is 329 amino acids long</jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Recombinant luciferase is not secreted when expressed in human cells</jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Exogenous luciferin does not seem to penetrate cell membranes-only lysate luminesces</jats:p>\n                      </jats:list-item>\n                      <jats:list-item>\n                        <jats:p>The luciferase transcript is supported by full-length cDNA reads with 5’ and 3’ UTR</jats:p>\n                      </jats:list-item>\n                    </jats:list>\n                  </jats:p>\n                  <jats:fig id=\"ufig1\" position=\"float\" fig-type=\"figure\" orientation=\"portrait\">\n                    <jats:graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"329631_ufig1\" position=\"float\" orientation=\"portrait\"/>\n                  </jats:fig>\n                </jats:sec>","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":null,"id":28366,"datarank":0.19580105927263505,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.031009215972418604,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.031009215972418604,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":1,"citers_with_citation_signal":1,"citers_with_endowment":1,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":159078,"name":"Alexey A. Kotlobay","orcid":null,"position":1,"is_corresponding":false},{"id":159079,"name":"Rustam Ziganshin","orcid":"0000-0002-7931-519X","position":2,"is_corresponding":false},{"id":159080,"name":"Artyom Bannikov","orcid":null,"position":3,"is_corresponding":false},{"id":159081,"name":"Nadezhda M. Markina","orcid":null,"position":4,"is_corresponding":false},{"id":159082,"name":"Tatiana V. Chepurnyh","orcid":null,"position":5,"is_corresponding":false},{"id":159083,"name":"Ekaterina S. Shakhova","orcid":null,"position":6,"is_corresponding":false},{"id":159084,"name":"Ksenia Palkina","orcid":null,"position":7,"is_corresponding":false},{"id":159085,"name":"Steven H.D. Haddock","orcid":null,"position":8,"is_corresponding":false},{"id":159086,"name":"Ilia V. Yampolsky","orcid":"0000-0003-2558-2476","position":9,"is_corresponding":false},{"id":159087,"name":"Yuichi Oba","orcid":null,"position":10,"is_corresponding":false},{"id":159077,"name":"Darrin T. Schultz","orcid":"0000-0003-1190-1122","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29792858","pmcid":null,"openalex_id":"https://openalex.org/W2803934923","authors":[],"funders":[{"funder_name":"National Science Foundation","grant_id":"1339067","title":"Gradute Research Fellowship Program (GRFP)"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":1,"citation_trend":[{"year":2018,"count":2}],"oa_status":"green","license":"cc-by","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2018/05/24/329631.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2018/05/24/329631.full.pdf","host_type":"GREEN"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2018/05/24/329631.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/329631","host_type":"publisher"},{"url":"https://doi.org/10.1101/329631","host_type":"repository"},{"url":"https://doi.org/10.1016/j.bbrc.2018.05.135","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/29792858","host_type":""},{"url":"https://dx.doi.org/10.1101/329631","host_type":""},{"url":"https://dx.doi.org/10.1016/j.bbrc.2018.05.135","host_type":""},{"url":"http://dx.doi.org/10.1101/329631","host_type":""}],"fields_of_study":["bioluminescence and chemiluminescence research","Tardigrade Biology and Ecology","Protist diversity and phylogeny","Biology","Chemistry","Medicine","Environmental Science","0301 basic medicine","0303 health sciences","0206 medical engineering","02 engineering and technology","03 medical and health sciences"],"mesh_terms":[],"keywords":["Luciferase","Bioluminescence","Luciferin","Biochemistry","Biology","Recombinant DNA","Complementary DNA","Luciferases","Polychaete","Chemistry","Gene","Transfection","Ecology","Luminescence","Sequence Homology, Amino Acid","Polychaeta","Recombinant Proteins","Evolution, Molecular","Japan","Structural Homology, Protein","Animals","Amino Acid Sequence"],"sdg_mappings":[{"sdg_number":14,"sdg_label":"14. 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