{"doi":"10.1101/2025.11.29.691293","title":"The Genomic Architecture of Human DNA Replication Origins","abstract":"Abstract The exact sites of DNA replication origins in human and other metazoans remain elusive. Examining human whole-genome sequencing data of 2,616 specimens, we observed conjoining reads at 2,025,756 non-random genomic positions, likely arising from nascent DNA and thus defining replication origins. These origins exhibited a 16 bp motif and periodic occurrence at the intervals of 10.5 bp and 200 bp. Genome-wide replication activity is related to the expression of DNA replication-related genes. Across the genome, DNA replication initiation is more active in early replicating regions, correlated with transcription activity in cis , enriched for de novo mutations and trait-associated polymorphisms. Our high-resolution mapping of human DNA replication origins points to molecular features that govern where and when replication begins in the genome.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":585377,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9234,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":262379,"name":"Wenjian Yang","orcid":"0000-0002-7305-5649","position":1,"is_corresponding":false},{"id":1204619,"name":"Gang Wu","orcid":"0000-0003-4379-7964","position":2,"is_corresponding":false},{"id":878579,"name":"Satoshi Yoshimura","orcid":"0000-0003-3490-2101","position":3,"is_corresponding":false},{"id":247450,"name":"Charles G. Mullighan","orcid":"0000-0002-1871-1850","position":4,"is_corresponding":false},{"id":423062,"name":"Tracie C. Rosser","orcid":null,"position":5,"is_corresponding":false},{"id":1403747,"name":"Elizabeth J. Leslie-Clarkson","orcid":null,"position":6,"is_corresponding":false},{"id":247452,"name":"Karen R. Rabin","orcid":"0000-0002-4081-8195","position":7,"is_corresponding":false},{"id":247448,"name":"Philip J. Lupo","orcid":"0000-0003-0978-5863","position":8,"is_corresponding":false},{"id":127468,"name":"David T. Teachey","orcid":null,"position":9,"is_corresponding":false},{"id":1459286,"name":"Jun J Yang","orcid":null,"position":10,"is_corresponding":false},{"id":301500,"name":"Zhenhua Li","orcid":"0000-0002-3623-6571","position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":null,"created_at":"2026-07-19T02:59:20.067334Z","pmid":"41394634","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}