{"doi":"10.1101/2025.11.24.690213","title":"A geothermal amoeba sets a new upper temperature limit for eukaryotes","abstract":"The study of temperature limits has transformed our knowledge of the boundaries of life but has been largely focused on bacteria and archaea. We isolated a novel geothermal amoeba, Incendiamoeba cascadensis, that divides at 63°C (145.4°F), establishing a new record for the upper temperature limit across all eukaryotes. We demonstrated cellular proliferation with growth experiments and visualized mitosis via expansion microscopy. Using high-temperature live-cell imaging, we quantified movement up to 64°C. We assembled the genome of I. cascadensis and using comparative genomics found an enrichment of genes related to proteostasis, genome stability, and sensing the external environment. Taken together, our findings challenge the current paradigm of temperature constraints on eukaryotic cells and reshape our understanding of where and how eukaryotic life can persist.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":555811,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9445,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1454681,"name":"Natalie A. Petek-Seoane","orcid":"0009-0003-8253-729X","position":1,"is_corresponding":false},{"id":1079832,"name":"Tomáš Tyml","orcid":"0000-0003-2558-9846","position":2,"is_corresponding":false},{"id":1454682,"name":"Felix Mikus","orcid":"0000-0002-5065-4710","position":3,"is_corresponding":false},{"id":296149,"name":"Kurt LaButti","orcid":"0000-0002-5838-1972","position":4,"is_corresponding":false},{"id":1333805,"name":"Godwin Ani","orcid":"0009-0008-8684-9844","position":5,"is_corresponding":false},{"id":394619,"name":"Jessica K. Niblo","orcid":"0000-0001-9378-6097","position":6,"is_corresponding":false},{"id":1454683,"name":"Ethan MacVicar","orcid":"0009-0002-7155-8223","position":7,"is_corresponding":false},{"id":1454684,"name":"Rachel M. Shepherd","orcid":"0000-0002-0795-4996","position":8,"is_corresponding":false},{"id":456848,"name":"Ignacio de la Higuera","orcid":"0000-0002-5457-9789","position":9,"is_corresponding":false},{"id":108494,"name":"Samuel J. Lord","orcid":"0000-0002-2785-989X","position":10,"is_corresponding":false},{"id":11059,"name":"Gautam Dey","orcid":"0000-0003-1416-6223","position":11,"is_corresponding":false},{"id":1454685,"name":"Gordon V. Wolfe","orcid":"0000-0003-2220-7590","position":12,"is_corresponding":false},{"id":1057013,"name":"Omaya Dudin","orcid":"0000-0002-6673-3149","position":13,"is_corresponding":false},{"id":559408,"name":"Shahar Sukenik","orcid":"0000-0003-3855-9574","position":14,"is_corresponding":false},{"id":428463,"name":"Laura A. Katz","orcid":"0000-0002-9138-4702","position":15,"is_corresponding":false},{"id":456868,"name":"Kenneth M. Stedman","orcid":"0000-0001-6086-0238","position":16,"is_corresponding":false},{"id":311454,"name":"Kristen Skruber","orcid":"0000-0003-2054-6652","position":17,"is_corresponding":false},{"id":628503,"name":"Frederik Schulz","orcid":"0000-0002-4932-4677","position":18,"is_corresponding":false},{"id":106699,"name":"R. Dyche Mullins","orcid":"0000-0002-0871-5479","position":19,"is_corresponding":false},{"id":1454686,"name":"Angela Oliverio","orcid":"0000-0002-0261-0393","position":20,"is_corresponding":false},{"id":1254927,"name":"Hannah Rappaport","orcid":"0000-0003-3106-4579","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:55:03.976486Z","pmid":"41394606","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}