{"doi":"10.1101/2025.11.06.686960","title":"Regulation of miR-219 and its target RalGPS dictates acute synaptic plasticity at the\n                  <i>Drosophila</i>\n                  neuromuscular junction","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  RNA regulatory mechanisms mediate adaptive responses requiring rapid gene expression changes. Examining microRNA (miRNA)-mediated control of structural synaptic plasticity, we identified miR-219 and six other conserved miRNAs regulating activity-induced morphogenesis at\n                  <jats:italic>Drosophila</jats:italic>\n                  glutamatergic synapses. Among miRNA targets downregulated by acute stimulation, the Ral-activating factor RalGPS is necessary for presynaptic morphogenesis and sufficient to block plasticity. Stimulation elevates miR-219 loading into Argonaute complexes without altering mature miR-219 levels, revealing a post-transcriptional regulatory mechanism. We conclude that activity-induced miRNA loading modulates Ral signaling via RalGPS to enable presynaptic remodeling and growth, establishing a novel link between neuronal activity and synaptic structural plasticity.\n                </jats:p>","journal":null,"year":null,"id":609231,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":350187,"name":"Hansine Heggeness","orcid":"0000-0002-1810-4073","position":1,"is_corresponding":false},{"id":1565760,"name":"Takakazu Yokokura","orcid":"0000-0002-0447-9739","position":2,"is_corresponding":false},{"id":302970,"name":"David Van Vactor","orcid":"0000-0001-8156-9482","position":3,"is_corresponding":false},{"id":1447909,"name":"Kumar Aavula","orcid":"0000-0002-7884-6730","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Regulation of miR-219 and its target RalGPS dictates acute synaptic plasticity at the\n                  <i>Drosophila</i>\n                  neuromuscular junction","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  RNA regulatory mechanisms mediate adaptive responses requiring rapid gene expression changes. Examining microRNA (miRNA)-mediated control of structural synaptic plasticity, we identified miR-219 and six other conserved miRNAs regulating activity-induced morphogenesis at\n                  <jats:italic>Drosophila</jats:italic>\n                  glutamatergic synapses. Among miRNA targets downregulated by acute stimulation, the Ral-activating factor RalGPS is necessary for presynaptic morphogenesis and sufficient to block plasticity. Stimulation elevates miR-219 loading into Argonaute complexes without altering mature miR-219 levels, revealing a post-transcriptional regulatory mechanism. We conclude that activity-induced miRNA loading modulates Ral signaling via RalGPS to enable presynaptic remodeling and growth, establishing a novel link between neuronal activity and synaptic structural plasticity.\n                </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"cc-by-nc","oa_locations":[{"url":"https://doi.org/10.1101/2025.11.06.686960","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2025.11.06.686960","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":[],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-31T04:55:08.327225Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}