{"doi":"10.1101/2025.11.06.682452","title":"3D Hybrid Bioprinting for Complex Multi-Tissue Engineering","abstract":"3D bioprinting has revolutionized tissue engineering, enabling intricate, physiologically relevant constructs unattainable with conventional techniques, yet it remains limited in integrating soft and rigid multifunctional components for complex multi-tissue applications. In this study, we introduce a 3D hybrid bioprinting approach implementing the Hybprinter platform, which integrates multiple 3D printing modules under optimized conditions for a continuous bioprinting process with multiple soft and hard biomaterials. This approach demonstrates robust biocompatibility and broad tissue engineering potential for modeling and therapeutic applications. The capacity to fabricate multi-hydrogel hybrid constructs is illustrated by representative examples highlighting vascularization, multifunctionality, mechanical robustness, and implant suturability. Notably, compared with commonly fabricated hydrogel-only constructs, the resulting hybrid constructs achieve over a 1000-fold increase in mechanical strength, and demonstrated enhanced osteogenic differentiation, underscoring their suitability for load-bearing musculoskeletal and orthopedic tissue engineering. Additionally, cell-laden hydrogel constructs demonstrated robust chondrogenic differentiation, highlighting the capacity for lineage-specific tissue development in vitro. Beyond these outcomes, the presented hybrid bioprinting approach integrates essential tissue engineering attributes that unites mechanical robustness and suturable capacity with multi-material integration, gradient property design, incorporation of bioactive agents, and support for multi-cell loading. This versatile platform advances complex tissue engineering and holds promise for patient specific, organ-on-demand applications.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":580422,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9519,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1383880,"name":"Andrea Sofia Flores Perez","orcid":"0009-0008-0137-9821","position":1,"is_corresponding":false},{"id":1491658,"name":"Tomohiro Uno","orcid":null,"position":2,"is_corresponding":false},{"id":1491659,"name":"Richard Sammy Muniz","orcid":null,"position":3,"is_corresponding":false},{"id":615461,"name":"Sun Hyung Kwon","orcid":"0000-0002-0455-2932","position":4,"is_corresponding":false},{"id":1491660,"name":"Anjana Balachandar","orcid":null,"position":5,"is_corresponding":false},{"id":1491661,"name":"Nicholas Riley","orcid":null,"position":6,"is_corresponding":false},{"id":1491662,"name":"Chantal Aivan Le","orcid":null,"position":7,"is_corresponding":false},{"id":407256,"name":"Jiannan Li","orcid":"0000-0002-6089-4785","position":8,"is_corresponding":false},{"id":666576,"name":"Peng Zhao","orcid":"0000-0003-3499-1415","position":9,"is_corresponding":false},{"id":368944,"name":"Elaine Lui","orcid":"0000-0001-7967-6596","position":10,"is_corresponding":false},{"id":368945,"name":"Carolyn Kim","orcid":"0000-0001-7355-7107","position":11,"is_corresponding":false},{"id":354458,"name":"Seyedsina Moeinzadeh","orcid":"0000-0002-4436-1807","position":12,"is_corresponding":false},{"id":1423895,"name":"Chi-Chun Pan","orcid":null,"position":13,"is_corresponding":false},{"id":233742,"name":"Nidhi Bhutani","orcid":"0000-0002-7494-5870","position":14,"is_corresponding":false},{"id":233740,"name":"Constance R. 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