{"doi":"10.1101/2025.11.05.685987","title":"Lateral plate mesoderm directs human amnion and ventral skin organoid formation","abstract":"Engineering organoids that faithfully replicate the intricate architecture and region-specific features of bodily organs and extraembryonic tissues remains a significant scientific challenge. Previously, we demonstrated that craniofacial skin organoids (cSkOs), containing epidermis, dermis, and hair, could be generated by co-developing epidermal progenitors with cranial mesenchyme. Building on this approach, we precisely adjusted cellular composition and signaling environments to generate ventral skin or-ganoids (vSkOs) with lateral plate mesoderm (LPM) progenitors, successfully recapitulating features of abdominal or groin skin. Modulating early BMP and FGF signaling redi-rected these vSkOs toward an extraembryonic fate, producing human amnion-like tis-sues, termed Amnioids. Like native human amnion, Amnioids rapidly expanded into large, avascular, hairless cysts, in sharp contrast to the primitive vasculature and abundant hair follicles of vSkOs. Single-cell RNA sequencing identified divergent molecular signatures and developmental trajectories, highlighting key roles for NOTCH, WNT, and YAP/Hippo signaling pathways. Functional studies further underscored mesenchymal-epithelial interactions and mechanical forces as critical regulators of epithelial expansion. Together, these models provide potent tools to investigate human development at the embryonic-extraembryonic interface, offering critical insights into congenital skin and amniotic disorders and opening new avenues for precision regenerative therapies.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":580321,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9579,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":847894,"name":"Jin Kim","orcid":"0000-0002-7667-9588","position":1,"is_corresponding":false},{"id":19583,"name":"Qianyi Ma","orcid":"0000-0002-6555-7446","position":2,"is_corresponding":false},{"id":1052897,"name":"Kelly Y Gim","orcid":"0009-0003-5634-6836","position":3,"is_corresponding":false},{"id":847893,"name":"Sara A. Serdy","orcid":"0000-0002-6076-9749","position":4,"is_corresponding":false},{"id":519741,"name":"Edward H. Lee","orcid":"0000-0003-2802-0786","position":5,"is_corresponding":false},{"id":1491145,"name":"Shariqa T Shaila","orcid":"0009-0003-2390-2189","position":6,"is_corresponding":false},{"id":1491146,"name":"Taiki Nakajima","orcid":"0000-0001-7420-5920","position":7,"is_corresponding":false},{"id":297709,"name":"Carl Nist-Lund","orcid":"0000-0002-0224-7387","position":8,"is_corresponding":false},{"id":104361,"name":"Ian A. Glass","orcid":"0000-0001-6762-8407","position":9,"is_corresponding":false},{"id":1491147,"name":"Yosuke Mai","orcid":"0000-0001-6356-7192","position":10,"is_corresponding":false},{"id":419977,"name":"Laura C. Nuzzi","orcid":"0000-0002-2236-6623","position":11,"is_corresponding":false},{"id":1007212,"name":"Catherine T. McNamara","orcid":null,"position":12,"is_corresponding":false},{"id":419976,"name":"Brian I. Labow","orcid":"0000-0002-7568-8634","position":13,"is_corresponding":false},{"id":669363,"name":"Liang Sun","orcid":"0000-0002-0321-9222","position":14,"is_corresponding":false},{"id":227672,"name":"Jiyoon Lee","orcid":"0000-0002-5561-5331","position":15,"is_corresponding":false},{"id":250294,"name":"Olivier Pourquié","orcid":"0000-0001-5189-1227","position":16,"is_corresponding":false},{"id":227679,"name":"Karl R. Koehler","orcid":"0000-0002-0685-538X","position":17,"is_corresponding":false},{"id":847895,"name":"Anh Phuong Le","orcid":"0000-0002-0477-9969","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:58:38.868285Z","pmid":"41278640","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}