{"doi":"10.1101/2025.10.13.682248","title":"Axially swept dithered light-sheet microscope to reveal cardiac morphology","abstract":"Understanding cardiac microstructure and vascular networks in their entirety is critical for assessing cardiovascular development, disease progression, and therapeutic interventions. Light-sheet microscopy combined with tissue clearing enables high-resolution volumetric imaging of intact organs but faces limitations in trabeculated myocardium due to trade-offs among light-sheet thickness, effective range, and frame rate. We exploit temporal dynamics that govern illumination-detection interplay to maintain uniform resolution across specimens. Building on this, we implemented high-speed dithered light-sheet (DiLS) illumination, extending the confocal region by over 40% and enhancing the space-bandwidth product while preserving optical sectioning. Integration of DiLS with a sweeping approach establishes the axially swept dithered light-sheet (AS-DiLS), which enhances imaging throughput while preserving axial resolution and enables uniform illumination up to 12.5-millimeter range. AS-DiLS delivers near-isotropic resolution (~2.5 μm) for investigating intricate ventricular trabeculae, vasculature, and extracellular matrix, providing a scalable platform for comprehensive cardiovascular morphology and topology assessment from embryos to adults. Teaser: Volumetric imaging reveals microstructure and vascular networks in their entirety with near-isotropic resolution.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":578081,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9584,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1106906,"name":"Alireza Saberigarakani","orcid":"0009-0008-7748-0424","position":1,"is_corresponding":false},{"id":961458,"name":"Xinyuan Zhang","orcid":"0009-0008-1914-3024","position":2,"is_corresponding":false},{"id":1488148,"name":"Jonathan A Brewer","orcid":null,"position":3,"is_corresponding":false},{"id":1488149,"name":"Rayaan Hameed","orcid":null,"position":4,"is_corresponding":false},{"id":1436787,"name":"Jichen Chai","orcid":null,"position":5,"is_corresponding":false},{"id":1487770,"name":"Yuanwei Lu","orcid":"0000-0002-2539-4566","position":6,"is_corresponding":false},{"id":809128,"name":"Sarah A. Ware","orcid":"0000-0002-2387-1613","position":7,"is_corresponding":false},{"id":1488150,"name":"Shuyue Zhou","orcid":null,"position":8,"is_corresponding":false},{"id":1477849,"name":"Yufeng Wen","orcid":"0000-0001-9338-918X","position":9,"is_corresponding":false},{"id":1488151,"name":"Hamza Lalami","orcid":null,"position":10,"is_corresponding":false},{"id":1488152,"name":"Hedyeh Hossein Beigi","orcid":null,"position":11,"is_corresponding":false},{"id":765438,"name":"Jie Yuan","orcid":"0000-0002-7789-6744","position":12,"is_corresponding":false},{"id":296179,"name":"Jun Liao","orcid":"0000-0003-1049-5783","position":13,"is_corresponding":false},{"id":1487771,"name":"Hong Yi","orcid":"0000-0002-7923-4971","position":14,"is_corresponding":false},{"id":309345,"name":"Mingfu Wu","orcid":"0000-0003-0155-2328","position":15,"is_corresponding":false},{"id":977565,"name":"Dan Tong","orcid":"0000-0003-3787-0707","position":16,"is_corresponding":false},{"id":529948,"name":"Darshan Sapkota","orcid":"0000-0003-3773-2910","position":17,"is_corresponding":false},{"id":385040,"name":"Yichen Ding","orcid":"0000-0002-6242-3506","position":18,"is_corresponding":false},{"id":1081824,"name":"Milad Almasian","orcid":"0009-0001-4159-8825","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-19T02:58:20.638044Z","pmid":"41279315","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}