{"doi":"10.1101/2025.10.01.679670","title":"AGO1 in neural progenitor cells orchestrates brain development and sociability via LIN28A-REELIN axis","abstract":"Abstract AGO1, an essential RNA binding protein (RBP) in RNA interference, is associated with autism spectrum disorder (ASD). However, the precise functions of AGO1 in brain development and related disorders remain largely unexplored. Here, we report the critical roles of AGO1 in neural progenitor cells (NPCs) in promoting sociability and shaping the structure of the developing brain. Ago1 knockout (KO) in the mouse brain leads to hyposociability, a characteristic symptom of ASD. In human forebrain organoids, AGO1 KO disrupts the formation of ventricle-like structures and delays cortical layer development. We discovered that AGO1 KO results in a loss of polarity in NPCs, which subsequently reduces neuronal development. These AGO1 KO phenotypes originate from the failure to suppress LIN28A in NPCs. AGO1 is primarily localized in the nucleus within NPCs and binds to the LIN28A promoter region, thereby inhibiting LIN28A transcription. We found that increased LIN28A in AGO1 KO NPCs reduces REELIN expression, a key regulator of brain development, by binding directly to the REELIN mRNA. The aberrant polarity phenotype in AGO1 KO NPCs was successfully ameliorated by either LIN28A knockdown or recombinant REELIN treatment. Collectively, our findings elucidate the intricate molecular mechanisms orchestrated by nuclear AGO1 in NPCs and underscores its pivotal roles in brain development, providing significant insights into ASD.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":576704,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9566,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1238656,"name":"Dayeon Kim","orcid":"0000-0003-4873-7493","position":1,"is_corresponding":false},{"id":978817,"name":"Roberto Jappelli","orcid":"0000-0002-0324-9085","position":2,"is_corresponding":false},{"id":1485534,"name":"Hyeok-Jun Yang","orcid":"0000-0003-0152-8520","position":3,"is_corresponding":false},{"id":1485946,"name":"Yi-Seon Jeon","orcid":null,"position":4,"is_corresponding":false},{"id":1238653,"name":"Geurim Son","orcid":"0000-0002-2470-8682","position":5,"is_corresponding":false},{"id":1238657,"name":"Mingyu Ju","orcid":"0000-0002-9879-7833","position":6,"is_corresponding":false},{"id":1238655,"name":"Ji-Hoon Son","orcid":"0000-0003-3233-039X","position":7,"is_corresponding":false},{"id":735182,"name":"In‐Sook Ahn","orcid":"0009-0004-7923-0788","position":8,"is_corresponding":false},{"id":280031,"name":"Christina Lim","orcid":null,"position":9,"is_corresponding":false},{"id":1485535,"name":"J. Ji","orcid":"0000-0002-5084-4805","position":10,"is_corresponding":false},{"id":1485536,"name":"Jihae Lee","orcid":"0009-0000-1431-229X","position":11,"is_corresponding":false},{"id":1295005,"name":"Young‐Kook Kim","orcid":"0000-0001-6434-2235","position":12,"is_corresponding":false},{"id":104394,"name":"Joon‐Yong An","orcid":"0000-0001-8839-6297","position":13,"is_corresponding":false},{"id":879714,"name":"Seung‐Hee Lee","orcid":"0000-0002-9486-5771","position":14,"is_corresponding":false},{"id":1052007,"name":"Daeyoup Lee","orcid":"0000-0003-2006-1823","position":15,"is_corresponding":false},{"id":249784,"name":"Fred H. Gage","orcid":"0000-0002-0938-4106","position":16,"is_corresponding":false},{"id":1238663,"name":"Jinju Han","orcid":"0000-0003-1844-2301","position":17,"is_corresponding":false},{"id":1239107,"name":"Hyunsu Do","orcid":null,"position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:58:00.620755Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}