{"doi":"10.1101/2025.09.22.677801","title":"Engineering Spatial Control of Bacterial Organelles","abstract":"Abstract Bacteria were once thought to lack organelles, but it is now clear they confine cellular reactions using an array of membrane- and protein-based compartments. A central question, however, is how bacterial organelles are organized in the cell, and whether their spatial control can be engineered. Here, we show that a two-protein system (McdAB) that positions carboxysomes-CO2-fixing organelles found in autotrophic bacteria-can be repurposed to provide programmable spatial control to diverse organelles in Escherichia coli . McdAB not only restores proper assembly and positioning of heterologously expressed carboxysomes in E. coli , but can also be reprogrammed to spatially organize all other known types of bacterial organelles, including encapsulins, biomolecular condensates, and even membrane-bound organelles. Programmable spatial organization of bacterial organelles establishes a new design principle for synthetic biology, where the location of reactions is as tunable as their content. Our work paves the way for more efficient biocatalysis in engineered microbes.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":575515,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9509,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1180555,"name":"Pankaj Jadhav","orcid":"0000-0003-0028-8975","position":1,"is_corresponding":false},{"id":909546,"name":"Daniel S. Trettel","orcid":"0000-0001-9206-4340","position":2,"is_corresponding":false},{"id":1071386,"name":"Rachel E. Dow","orcid":null,"position":3,"is_corresponding":false},{"id":1004334,"name":"Seokmu Kwon","orcid":"0000-0002-0111-5219","position":4,"is_corresponding":false},{"id":1483784,"name":"Kate Matej","orcid":null,"position":5,"is_corresponding":false},{"id":1043200,"name":"Jordan A. Byrne","orcid":"0000-0002-9480-6587","position":6,"is_corresponding":false},{"id":242839,"name":"Christopher A. Azaldegui","orcid":"0000-0002-3889-2660","position":7,"is_corresponding":false},{"id":470992,"name":"Tobias W. Giessen","orcid":"0000-0001-6328-2031","position":8,"is_corresponding":false},{"id":338745,"name":"Hualiang Pi","orcid":"0000-0002-0015-4443","position":9,"is_corresponding":false},{"id":474533,"name":"Shyamal Mosalaganti","orcid":"0000-0002-5934-687X","position":10,"is_corresponding":false},{"id":242840,"name":"Anthony G. Vecchiarelli","orcid":"0000-0002-6198-3245","position":11,"is_corresponding":false},{"id":1043201,"name":"Y Hoang","orcid":"0000-0001-8804-534X","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:57:48.486077Z","pmid":"41040230","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}