{"doi":"10.1101/2025.09.18.676951","title":"A protocol for high-throughput microplate-based CUT&Tag","abstract":"Abstract Cleavage Under Targets & Tagmentation (CUT&Tag) is a versatile method for measuring genomic occupancy of chromatin-associated proteins with high sensitivity and specificity. CUT&Tag has low sequencing requirements and is therefore suitable for highly multiplexed experiments, but methods to process samples at throughput without specialized equipment are lacking. Here we present a method for simultaneous parallel processing of 96 CUT&Tag samples in a standard microplate. Plate-CUT&Tag can be carried out in a similar time frame to benchtop CUT&Tag and yields data of comparable quality. We present data from cell culture and patient leukemia samples processed with Plate-CUT&Tag to illustrate its utility in large-scale preclinical and translational studies.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":575795,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9618,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":633092,"name":"Gabriel Boyle","orcid":"0000-0002-2019-5787","position":1,"is_corresponding":false},{"id":987503,"name":"Arnold J Federico","orcid":"0000-0001-6135-9090","position":2,"is_corresponding":false},{"id":410054,"name":"Jing Ma","orcid":"0000-0003-1098-945X","position":3,"is_corresponding":false},{"id":860993,"name":"Humza Hemani","orcid":"0000-0003-1766-683X","position":4,"is_corresponding":false},{"id":725563,"name":"David H. Spencer","orcid":"0000-0002-5314-3043","position":5,"is_corresponding":false},{"id":267024,"name":"Jay F. Sarthy","orcid":"0000-0001-5244-7865","position":6,"is_corresponding":false},{"id":267025,"name":"Michael P. Meers","orcid":"0000-0003-3438-3938","position":7,"is_corresponding":false},{"id":1484378,"name":"Brittany B. Johnson","orcid":null,"position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":null,"created_at":"2026-07-19T02:57:52.712371Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}