{"doi":"10.1101/2025.08.27.672012","title":"ESPWA: a deep learning tool to inform precision-based use of endocrine therapy in resource-limited settings","abstract":"Background: nalysis), a deep-learning (DL) model that predicts ER status directly from hematoxylin-and-eosin (H&E)-stained whole slide images (WSIs). Methods: We curated two cohorts of H&E WSIs with tissue-matched ER status: The Cancer Genome Atlas (TCGA, n = 1085) and Zanmi Lasante (ZL, n = 3448) from Haiti. We trained two models using weakly supervised attention-based multiple instance learning: a \"TCGA\" model, trained on TCGA data, and ESPWA, trained on the ZL dataset. Model performance was evaluated using 10-fold cross validation. Results: Performance of the \"TCGA\" model was sensitive to the domain shift between the TCGA and ZL datasets, with a performance of an area under receiver operating characteristic (AUROC) of 0.846 on the TCGA test sets and 0.671 on the ZL test sets. Compared to the \"TCGA\" model, ESPWA demonstrated improved performance on the ZL cohort (AUROC=0.790; p=0.005). Subgroup analyses revealed clinically relevant populations in which ESPWA demonstrated improved performance relative to the overall cohort. Finally, ESPWA outperformed an academic breast pathologist (accuracy: 0.726 vs 0.639 respectively; p <0.001) in determining ER status from H&E WSIs. Conclusion: ESPWA (\"Hope\" in Haitian Creole) offers an accessible framework to identify individualized therapeutic insights from H&E WSIs in LMICs. We have initiated clinical trials, using ESPWA, in ZL and sub-Saharan African countries to inform precision-based use of ET for prospective patients.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":573626,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8421,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":430587,"name":"Rebecca Henderson","orcid":"0000-0003-2142-3566","position":1,"is_corresponding":false},{"id":1021076,"name":"Christophe Millien","orcid":"0000-0001-8590-812X","position":2,"is_corresponding":false},{"id":1481175,"name":"Mervin José","orcid":null,"position":4,"is_corresponding":false},{"id":1481176,"name":"Gabriel Flambert","orcid":null,"position":5,"is_corresponding":false},{"id":1481177,"name":"Jean Bontemps","orcid":null,"position":7,"is_corresponding":false},{"id":1481178,"name":"Gunturi Alekhya","orcid":null,"position":8,"is_corresponding":false},{"id":297886,"name":"Palak Shah","orcid":"0000-0001-8175-168X","position":9,"is_corresponding":false},{"id":1480802,"name":"Tiago Gonçalves","orcid":"0000-0002-1031-0032","position":11,"is_corresponding":false},{"id":14554,"name":"Jayashree Kalpathy-Cramer","orcid":null,"position":12,"is_corresponding":false},{"id":247458,"name":"Elizabeth R. Gerstner","orcid":"0000-0003-2382-3838","position":13,"is_corresponding":false},{"id":3745,"name":"Seth A. Wander","orcid":"0000-0002-4127-4708","position":14,"is_corresponding":false},{"id":250485,"name":"S. Joseph Sirintrapun","orcid":"0000-0003-2921-8831","position":15,"is_corresponding":false},{"id":14035,"name":"Dennis C. Sgroi","orcid":"0000-0003-4052-6659","position":16,"is_corresponding":false},{"id":336967,"name":"José Jerónimo","orcid":"0000-0002-7734-1837","position":17,"is_corresponding":false},{"id":285993,"name":"Philip E. Castle","orcid":"0000-0002-6408-5985","position":18,"is_corresponding":false},{"id":1480803,"name":"Kenneth Landgraf","orcid":"0000-0001-5462-0561","position":19,"is_corresponding":false},{"id":1480804,"name":"Ali Brown","orcid":"0000-0002-3679-1188","position":20,"is_corresponding":false},{"id":582241,"name":"Temidayo Fadelu","orcid":"0000-0002-1323-4119","position":21,"is_corresponding":false},{"id":263415,"name":"Lawrence N. Shulman","orcid":"0000-0001-8661-473X","position":22,"is_corresponding":false},{"id":89118,"name":"John Guttag","orcid":null,"position":23,"is_corresponding":false},{"id":995043,"name":"Jane B. Brock","orcid":"0000-0003-3358-0534","position":24,"is_corresponding":false},{"id":1481179,"name":"Christopher P Bridge","orcid":null,"position":26,"is_corresponding":false},{"id":463319,"name":"Albert E. Kim","orcid":"0000-0003-2359-6209","position":27,"is_corresponding":false},{"id":1481174,"name":"Dagoberto Pulido-Arias","orcid":null,"position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":null,"created_at":"2026-07-19T02:57:36.753600Z","pmid":"40909747","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}