{"doi":"10.1101/2025.08.14.670421","title":"Analyzing the role of a dual-function diguanylate cyclase/phosphodiesterase for attachment and motility in <i>Agrobacterium tumefaciens</i>","abstract":"Abstract Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger that controls the signaling pathway for the motile to sessile transition in many bacteria. Diguanylate cyclases (DGCs), characterized by a GGDEF domain, are responsible for the synthesis of c-di-GMP. c-di-GMP is degraded by EAL and HD-GYP domains that exhibit phosphodiesterase activity (PDE). In Agrobacterium tumefaciens , out of 31 predicted proteins regulating the c-di-GMP levels, this work focuses on the predicted dual-function DGC/PDE, DcpB. We show that DcpB is a cycle-dependent PDE under our experimental conditions, resulting in cell cycle-dependent control of motility and biofilm formation in A. tumefaciens . DcpB also exhibits polar and mid-cell localization. Finally, we identify genetic interactions between dcpB and a LacI-family transcriptional regulators, thuR , suggesting additional regulatory inputs for DcpB-dependent phenotypes. Importance c-di-GMP is a universal secondary messenger known to control various biological processes in bacteria including attachment, motility, virulence, and cell cycle progression. In A. tumefaciens , there are 31 predicted c-di-GMP-metabolizing proteins predicted to affect the c-di-GMP pool, raising the question of why so many proteins are involved and how the activities of these proteins are coordinated. This work describes a cell cycle-dependent PDE activity that helps coordinate cell cycle progression and developmental phenotypes including biofilm formation and motility.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":572827,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9613,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":530366,"name":"Matthew P. Anderson","orcid":"0000-0003-4602-1811","position":1,"is_corresponding":false},{"id":1479715,"name":"Hannah Turrisi","orcid":null,"position":2,"is_corresponding":false},{"id":981479,"name":"Aathmaja Anandhi Rangarajan","orcid":"0000-0003-3471-0257","position":3,"is_corresponding":false},{"id":1479716,"name":"Mackenzie Brooks","orcid":null,"position":4,"is_corresponding":false},{"id":1169053,"name":"Gianna M. Rodriguez","orcid":"0000-0002-1655-9402","position":5,"is_corresponding":false},{"id":1245450,"name":"Delayna L. Warrell","orcid":null,"position":6,"is_corresponding":false},{"id":1479717,"name":"Anthony Pangalinan","orcid":null,"position":7,"is_corresponding":false},{"id":1479718,"name":"Ganishah Charles-Estain","orcid":null,"position":8,"is_corresponding":false},{"id":1479719,"name":"Ravinder Abraham","orcid":null,"position":9,"is_corresponding":false},{"id":1479720,"name":"Hope Zaborowski","orcid":null,"position":10,"is_corresponding":false},{"id":1479721,"name":"Dalal Seyhan","orcid":null,"position":11,"is_corresponding":false},{"id":400506,"name":"Christopher M. Waters","orcid":"0000-0003-2336-7836","position":12,"is_corresponding":false},{"id":413845,"name":"Jason E. Heindl","orcid":"0000-0003-3919-8045","position":13,"is_corresponding":false},{"id":1479714,"name":"Priya Aryal","orcid":null,"position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-19T02:57:27.876396Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}