{"doi":"10.1101/2025.08.06.668980","title":"A modular method for rapidly prototyping targeted gas vesicle protein nanoparticles","abstract":"Abstract Gas vesicles (GVs) are air-filled protein nanoparticles which are proving to be useful in a number of biomedical applications. We hypothesized that it could be possible to develop a modular method for creating rapidly prototyped GVs by modifying their surface chemistry to include targeting peptides in an orientation-specific manner. Here, we describe a modular method to create targeted GVs using His-tagged antibody fragments, ensuring that the antibody fragments are connected to the GV in an orientation-specific manner. This is achieved via the functionalization of the GVs with nickel-nitrilotriacetic acid (Ni-NTA) group. First, we validated that these functionalized GVs can bind His-tagged green fluorescent protein and characterized the particle size and surface charge of functionalized GVs. Then, GVs targeted to prostate-specific membrane antigen (PSMA) using a minibody were validated using a knockout validation in vitro .","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":572246,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9474,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":237832,"name":"Bill Ling","orcid":"0000-0002-1276-7204","position":1,"is_corresponding":false},{"id":667823,"name":"Ernesto Criado-Hidalgo","orcid":"0000-0001-9086-9129","position":2,"is_corresponding":false},{"id":1478443,"name":"Nicole Robinson","orcid":"0000-0001-6285-6179","position":3,"is_corresponding":false},{"id":1179156,"name":"Erik Schrunk","orcid":null,"position":4,"is_corresponding":false},{"id":289195,"name":"Ann Liu","orcid":"0000-0002-0908-3506","position":5,"is_corresponding":false},{"id":331859,"name":"George Daghlian","orcid":"0000-0002-4921-0442","position":6,"is_corresponding":false},{"id":237830,"name":"Hongyi Li","orcid":"0000-0001-6970-0230","position":7,"is_corresponding":false},{"id":310821,"name":"M Swift","orcid":"0000-0001-9610-0687","position":8,"is_corresponding":false},{"id":267228,"name":"Dhiraj Mannar","orcid":"0000-0002-7154-8677","position":9,"is_corresponding":false},{"id":258875,"name":"Dina Malounda","orcid":"0000-0001-7086-9877","position":10,"is_corresponding":false},{"id":715190,"name":"S. Larry Goldenberg","orcid":"0000-0002-0572-4206","position":11,"is_corresponding":false},{"id":913091,"name":"Septimiu E. Salcudean","orcid":"0000-0001-8826-8025","position":12,"is_corresponding":false},{"id":237833,"name":"Mikhail G. Shapiro","orcid":"0000-0002-0291-4215","position":13,"is_corresponding":false},{"id":405502,"name":"Peter Black","orcid":null,"position":14,"is_corresponding":false},{"id":1478444,"name":"Michael Cox","orcid":"0000-0001-6468-6046","position":15,"is_corresponding":false},{"id":1011933,"name":"Reid Vassallo","orcid":null,"position":0,"is_corresponding":true}],"reference_count":26,"raw_metadata":null,"created_at":"2026-07-19T02:57:23.653298Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}