{"doi":"10.1101/2025.05.23.25328123","title":"COVID-19 Vaccination Induces Cross-Reactive Dengue Antibodies with Altered Isotype Profiles and In Vitro ADE","abstract":"Abstract Dengue virus is a mosquito transmitted flavivirus that cause inapparent or mild disease but can also cause severe hemorrhagic fever in humans. Cross-reactive IgG antibodies can cause under specific conditions antibody dependent enhancement (ADE) of the infection via Fc-receptor interaction. Antibodies from SARS-CoV-2 (anti-S) causing COVID-19 disease can also cross-react against the dengue envelope (anti-E). We therefore investigated the antibody profile of these cross-reactive antibodies and their ability to induce ADE. We found that the cross-reactive anti-E in COVID-19 vaccinated are dominated by IgM/A while, anti-S from vaccinated or anti-E from dengue infected patients have an IgG1 dominated antibody profile. The low-level anti-E IgG from COVID-19 vaccinated are dominated by low Fc-affinity IgG2/4 subclass. These antibodies were able to induce in vitro ADE stronger than from Dengue infected for the 1 st ,2 nd dose of the vaccine or later omicron variant booster. This could partially be explained that ADE was inhibited by the complement system in dengue infected but not in COVID-19 vaccinated.","journal":"medRxiv","year":2025,"id":556355,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1188649,"name":"Chin Hwa Kuo","orcid":null,"position":1,"is_corresponding":false},{"id":713309,"name":"Sheng‐Yu Huang","orcid":"0000-0002-7626-688X","position":2,"is_corresponding":false},{"id":439332,"name":"Kuei-Ching Hsiung","orcid":"0000-0002-2107-6368","position":3,"is_corresponding":false},{"id":1443705,"name":"Po-Kai Chen","orcid":"0009-0006-7830-0590","position":4,"is_corresponding":false},{"id":120069,"name":"Etsuro Ito","orcid":"0000-0002-1877-6566","position":5,"is_corresponding":false},{"id":923057,"name":"Ing‐Kit Lee","orcid":null,"position":6,"is_corresponding":false},{"id":1443706,"name":"Ching‐Yen Tsai","orcid":"0000-0001-7964-6661","position":7,"is_corresponding":false},{"id":922474,"name":"Shu‐Min Lin","orcid":"0000-0002-0834-564X","position":8,"is_corresponding":false},{"id":559167,"name":"Shin‐Ru Shih","orcid":"0000-0003-4874-401X","position":9,"is_corresponding":false},{"id":1070082,"name":"Sebastian Reinig","orcid":null,"position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-19T02:55:08.896385Z","pmid":"40661288","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}