{"doi":"10.1101/2025.05.18.654753","title":"Backbone Assignment of a 28.5 kDa Class A Extended Spectrum β-Lactamase by High-Field, Carbon-Detected Solid-State NMR","abstract":"N backbone chemical shift assignments are reported for the 28.5 kDa protein Toho-1 β-lactamase, a Class A extended spectrum β-lactamase. A very high level of assignment completeness (97% of the backbone) is enabled by the combined sensitivity and resolution gains of ultrahigh-field NMR spectroscopy (1.1 GHz), improved probe technology, and optimized pulse sequences. The assigned chemical shifts agree well with our previous solution-state NMR assignments, indicating that the secondary structure is conserved in the solid state. These assignments provide a foundation for future investigations of sidechain chemical shifts and catalytic mechanism.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":566370,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9138,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":418593,"name":"Songlin Wang","orcid":"0000-0002-7588-7377","position":1,"is_corresponding":false},{"id":1436868,"name":"Alexander F. Thome","orcid":null,"position":2,"is_corresponding":false},{"id":707637,"name":"Owen A. Warmuth","orcid":"0000-0001-9731-5487","position":3,"is_corresponding":false},{"id":455591,"name":"Varun V. Sakhrani","orcid":null,"position":4,"is_corresponding":false},{"id":526696,"name":"Chad M. Rienstra","orcid":"0000-0002-9912-5596","position":5,"is_corresponding":false},{"id":454378,"name":"Leonard J. Mueller","orcid":"0000-0002-2607-9875","position":6,"is_corresponding":false},{"id":1405312,"name":"Christopher G. Williams","orcid":"0009-0009-8319-4413","position":0,"is_corresponding":true}],"reference_count":46,"raw_metadata":null,"created_at":"2026-07-19T02:56:36.440192Z","pmid":"40475586","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}