{"doi":"10.1101/2025.05.15.653195","title":"Peptide nucleic acid (PNA) and DNA hybrid three-way junctions and mesojunctions","abstract":"Three-way junctions are simple and fundamental structural motifs that impart the typical branching property in most DNA nanostructures. While conventional three-way DNA junctions are well-known, mesojunctions are relatively unexplored. Here, we report the synthesis of peptide nucleic acid (PNA)/DNA hybrid three-way conventional and mesojunctions, containing a 14 bp duplex DNA arm and two PNA/DNA hybrid arms. We show that the PNA/DNA mesojunction can be assembled in magnesium-free buffers containing low concentrations of calcium or sodium salts. PNA/DNA hybrid junctions and mesojunctions showed higher thermal stability compared to the DNA versions. Further, PNA/DNA hybrid junctions assembled in sodium exhibited higher nuclease resistance against DNase I. Our results pave the way for PNA/DNA hybrid three-way junctions to be integrated into complex DNA nanostructure designs to improve their structural and enzymatic stability.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":555560,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9608,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":966444,"name":"Akul Patel","orcid":null,"position":1,"is_corresponding":false},{"id":906904,"name":"Hannah Talbot","orcid":"0009-0002-5229-2220","position":2,"is_corresponding":false},{"id":1454340,"name":"John A. Geary","orcid":null,"position":3,"is_corresponding":false},{"id":1453830,"name":"Krishna N. Ganesh","orcid":"0000-0003-2292-643X","position":4,"is_corresponding":false},{"id":286364,"name":"Arun Richard Chandrasekaran","orcid":"0000-0001-6757-5464","position":5,"is_corresponding":false},{"id":1035109,"name":"Bharath Raj Madhanagopal","orcid":null,"position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-19T02:54:59.329539Z","pmid":"40463137","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}