{"doi":"10.1101/2025.05.12.653583","title":"Non-redundant cardiolipin synthases shape lipid composition and stress resilience in <i>Bacteroides fragilis</i>","abstract":"Abstract Gut-resident bacteria must tolerate diverse membrane-disrupting agents, including bile acids, to maintain colonization. Cardiolipin is an anionic phospholipid that supports membrane integrity and stress resilience in many bacteria. However, cardiolipin synthases remain poorly characterized in the Bacteroidota, a dominant phylum of the human gut microbiota. The prevalent gut commensal Bacteroides fragilis encodes two predicted cardiolipin synthases, ClsA and ClsB. We previously identified both cls genes as bile-acid fitness factors in B. fragilis P207, but the individual contributions of ClsA and ClsB to cell physiology had remained undefined. Here we combine targeted gene deletion with high-resolution lipidomics, metabolomics, and elemental mass spectrometry to show that the two enzymes have non-redundant functions in the cell. Cardiolipin is a minor lipid in the B. fragilis membrane, and both Cls enzymes contribute to its production. clsA and clsB differ in growth-phase expression, in their effects on cell morphology, and in their associated cardiolipin species. Loss of each enzyme also produces distinct changes in fitness under several membrane-perturbing stresses and in the broader cellular metabolome, including compound classes with documented bioactivity in mammalian hosts. In contrast to the acute ion-gradient disruption caused by the secondary bile acid deoxycholate, deletion of both synthases did not measurably alter steady-state intracellular ion levels under standard growth conditions, indicating that cardiolipin loss does not perturb basal ion homeostasis under these conditions. Together, these results define non-redundant roles for two cardiolipin synthases in a common member of the human gut microbiota. Importance Inflammatory bowel diseases affect millions of people worldwide. The gut bacterium Bacteroides fragilis is a normal member of the human intestinal microbiota that can also bloom to high abundance in inflamed guts. To survive within the gut, B. fragilis must maintain the integrity and function of its cell membrane. In this study, we characterize the functional role of two B. fragilis genes that contribute to the synthesis of the membrane lipid, cardiolipin. We find that the two cardiolipin synthase genes are not functionally interchangeable; each impacts the cell’s lipid pool in distinct ways and contributes differently to how B. fragilis responds to membrane stress. Our work provides insight into how a common gut bacterium adapts to conditions encountered in the intestine, and improves understanding of membrane biology in this important group of gut microbes.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":565830,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9588,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1469283,"name":"Bum‐Soo Hong","orcid":"0000-0003-2992-9617","position":1,"is_corresponding":false},{"id":1469727,"name":"Jennifer N.T. Nguyen","orcid":null,"position":2,"is_corresponding":false},{"id":881015,"name":"Katarina A. Jones","orcid":"0000-0003-3769-8599","position":3,"is_corresponding":false},{"id":1469728,"name":"Alyssa I. Rodriguez","orcid":null,"position":4,"is_corresponding":false},{"id":365978,"name":"Aretha Fiebig","orcid":"0000-0002-0612-5029","position":5,"is_corresponding":false},{"id":621104,"name":"Shawn R. Campagna","orcid":"0000-0001-6809-3862","position":6,"is_corresponding":false},{"id":235442,"name":"Marcy J. Balunas","orcid":"0000-0003-2374-4048","position":7,"is_corresponding":false},{"id":361448,"name":"Thomas V. O’Halloran","orcid":"0000-0001-8732-5059","position":8,"is_corresponding":false},{"id":365979,"name":"Sean Crosson","orcid":"0000-0002-1727-322X","position":9,"is_corresponding":false},{"id":816987,"name":"Matthew K. Schnizlein","orcid":"0000-0002-0797-8357","position":0,"is_corresponding":true}],"reference_count":152,"raw_metadata":null,"created_at":"2026-07-19T02:56:32.546082Z","pmid":"40463285","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}