{"doi":"10.1101/2025.05.08.652883","title":"Characterization of the glycoproteins of novel fish influenza B-like viruses","abstract":"Novel influenza-like virus sequences previously identified in fish and amphibians were found to cluster as a sister clade of influenza B viruses, but have thus far remained uncharacterized. We demonstrate that salamander influenza-like virus (SILV) HA is functionally divergent from influenza B virus HA and does not bind to α 2,3- and α2,6-linked sialic acids. However, the HAs of Siamese algae-eater influenza-like virus (SAEILV) and chum salmon influenza-like virus (CSILV) bind to α2,3 linked sialic acid. Furthermore, SAEILV HA binds to sialyated Lewis X, is activated by human airway enzymes and is fusogenic at a wide range of pH conditions. SAEILV NA has a highly conserved active site and a similar structure to other known NAs. We also determined the cryo-electron microscopy structure of the HA of a previously described virus from the same sister clade, the Wuhan spiny eel influenza virus (WSEIV). Importantly, no cross-reactive antibodies against these HAs or NAs were found in the human serum, suggesting that humans are immunologically naïve to these viruses.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":565648,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9604,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":355921,"name":"Jiachen Huang","orcid":"0000-0001-7624-7390","position":1,"is_corresponding":false},{"id":805371,"name":"Disha Bhavsar","orcid":"0000-0003-1564-3298","position":2,"is_corresponding":false},{"id":1450816,"name":"Kirill Vasilev","orcid":"0000-0002-7750-9652","position":3,"is_corresponding":false},{"id":919707,"name":"James A. Ferguson","orcid":"0000-0003-2891-0850","position":4,"is_corresponding":false},{"id":53134,"name":"Geert‐Jan Boons","orcid":"0000-0003-3111-5954","position":5,"is_corresponding":false},{"id":104936,"name":"Viviana Simon","orcid":"0000-0002-6416-5096","position":6,"is_corresponding":false},{"id":472820,"name":"Robert P. de Vries","orcid":"0000-0002-1586-4464","position":7,"is_corresponding":false},{"id":284389,"name":"Julianna Han","orcid":"0000-0002-3402-2609","position":8,"is_corresponding":false},{"id":230082,"name":"Andrew B. Ward","orcid":"0000-0001-7153-3769","position":9,"is_corresponding":false},{"id":103779,"name":"Florian Krammer","orcid":"0000-0003-4121-776X","position":10,"is_corresponding":false},{"id":262427,"name":"Gagandeep Singh","orcid":"0000-0001-6661-3553","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-19T02:56:32.546082Z","pmid":"40654808","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}