{"doi":"10.1101/2025.05.07.652694","title":"Pln1 Mediates Lipid Droplet-Vacuole Tethering During Microlipophagy in <i>Saccharomyces cerevisiae</i>","abstract":"Abstract Lipid droplets (LDs) are dynamic organelles that undergo growth or degradation depending on the metabolic state of the cell. One form of LD degradation is autophagy-mediated, referred to as lipophagy. Here, we demonstrate that Pln1, a perilipin located on the surface of LDs in Saccharomyces cerevisiae , previously known for its role in LD biogenesis, is essential for lipophagy. Pln1 facilitates the docking of cytosolic LDs to vacuoles, the lysosome-like organelles responsible for LD degradation, under various nutrient conditions. Molecular dissection of Pln1 revealed that the N-terminal PAT (Perilipin (PLN1), Adipophilin (PLN2), and TIP47 (PLN3)) domain and a hydrophobic region are critical for the localization and binding of LDs to vacuoles. Site-specific mutagenesis within the PAT domain identified a semi-hydrophobic LD Interacting Motif (LIM), which is vital for this interaction. Furthermore, an intrinsically disordered region (IDR) near the center of Pln1 is required for efficient LD-vacuole tethering. These findings support a model in which Pln1 bridges LDs and vacuoles by simultaneously interacting with both organelles. Notably, deleting PLN1 did not impair survival during prolonged nitrogen starvation and enhanced viability in autophagy-defective ( atg8 Δ) cells, suggesting that balancing Pln1-mediated LD biogenesis and lipophagy is crucial for yeast survival under starvation conditions.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":556297,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":808463,"name":"Brayden Folger","orcid":null,"position":1,"is_corresponding":false},{"id":462641,"name":"Joshua B. Reus","orcid":null,"position":2,"is_corresponding":false},{"id":689011,"name":"Alexandre Toulmay","orcid":"0009-0009-5383-3632","position":3,"is_corresponding":false},{"id":272532,"name":"Jin Li","orcid":"0000-0001-9201-2321","position":4,"is_corresponding":false},{"id":1129463,"name":"Rudian Zhang","orcid":"0000-0003-2214-4892","position":5,"is_corresponding":false},{"id":444294,"name":"William A. Prinz","orcid":"0000-0002-9053-2398","position":6,"is_corresponding":false},{"id":542347,"name":"Joel Goodman","orcid":"0000-0001-5483-202X","position":7,"is_corresponding":false},{"id":828661,"name":"Fei Wang","orcid":"0000-0001-7899-244X","position":8,"is_corresponding":false},{"id":664245,"name":"Monala Jayaprakash Rao","orcid":null,"position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:55:08.896385Z","pmid":"40463259","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}