{"doi":"10.1101/2025.05.07.652656","title":"mtgRNA-db: An annotated database of multi-target CRISPR-Cas9 guide-RNAs in the human genome","abstract":"CRISPR-Cas9 guide-RNA design tools can be used to identify guide-RNAs that target single human genomic loci. However, these approaches limit their effect to a single locus. Here, we generate a database of potential individual guide-RNAs that target multiple sites in the genome at once. All guide-RNAs in this database are curated with on- and off-target quantification and enrichment scores for genomic elements such as gene elements, regulatory elements, repetitive elements, and transcription factor motifs. This tool enables rationale mass targeting of genomic loci with a single guide for functional studies. We created a web-app for user-friendly guide-RNA selection (https://modreklab.shinyapps.io/guiderna/).","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":557837,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":22.178386323199504,"corpus_rank":9377,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.95,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1007750,"name":"Khoi Huynh","orcid":"0009-0002-5428-753X","position":1,"is_corresponding":false},{"id":322441,"name":"Catherine Do","orcid":"0000-0002-9796-4345","position":2,"is_corresponding":false},{"id":1257273,"name":"Jerome M. Karp","orcid":"0000-0001-7266-0946","position":3,"is_corresponding":false},{"id":945118,"name":"Yingwen Ding","orcid":"0000-0001-7673-8499","position":4,"is_corresponding":false},{"id":945119,"name":"Ze-Yan Zhang","orcid":"0000-0002-7896-3013","position":5,"is_corresponding":false},{"id":945117,"name":"Ravesanker Ezhilarasan","orcid":"0000-0002-1353-8307","position":6,"is_corresponding":false},{"id":1458320,"name":"Belen Valor","orcid":null,"position":7,"is_corresponding":false},{"id":1327859,"name":"Giulia Cova","orcid":"0000-0001-6489-8807","position":8,"is_corresponding":false},{"id":238127,"name":"Jane A. Skok","orcid":"0000-0002-4145-1516","position":9,"is_corresponding":false},{"id":275936,"name":"Erik P. Sulman","orcid":"0000-0003-4933-9120","position":10,"is_corresponding":false},{"id":304305,"name":"Aram S. Modrek","orcid":"0000-0001-7586-9833","position":0,"is_corresponding":true}],"reference_count":24,"raw_metadata":null,"created_at":"2026-07-19T02:55:21.727661Z","pmid":"40654676","pmcid":"PMC12247669","fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}