{"doi":"10.1101/2025.04.27.650873","title":"Comparative Virome Profiling and Discovery of Novel Viruses in Managed and Wild Bees using RNA-seq","abstract":"Abstract Insect pollinators face growing challenges from viral pathogens that may contribute to population declines, yet comparative data on virome composition in managed versus wild bees remain limited. Using RNA-Seq-based metatranscriptomic analysis, we compared the virome of Apis mellifera L. and Ceratina calcarata Robertson collected from agricultural and non-agricultural landscapes. We identified multiple known bee pathogens including Black Queen Cell Virus and Deformed Wing Virus in A. mellifera , and discovered three novel insect viruses—two iflaviruses and one entomopoxvirus—associated with C. calcarata . Notably, one novel iflavirus was identified in A. mellifera . Our findings reveal species- and landscape-specific viral prevalence, highlight viral diversity beyond canonical bee pathogens, and provide genomic resources for future functional studies. These data offer new insights into virus-host dynamics and underscore the utility of virome profiling in understanding pollinator health at the molecular level.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":565099,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.916,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1468645,"name":"Mary Farrell","orcid":"0000-0002-0081-3285","position":1,"is_corresponding":false},{"id":1468646,"name":"Rameshwor Pudasaini","orcid":"0000-0001-9957-1185","position":2,"is_corresponding":false},{"id":1468902,"name":"Jayla Marvin","orcid":null,"position":3,"is_corresponding":false},{"id":1468647,"name":"Sandra M. Rehan","orcid":"0000-0002-6441-5155","position":4,"is_corresponding":false},{"id":1449137,"name":"Hongmei Li‐Byarlay","orcid":"0000-0001-6579-5172","position":5,"is_corresponding":false},{"id":1468644,"name":"Zixiao Zhao","orcid":"0000-0002-3627-5503","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T02:56:28.709936Z","pmid":"40463272","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}