{"doi":"10.1101/2025.04.23.650263","title":"Developmental dynamics and axonal transport of cerebral cortex projection neuron mRNAs <i>in vivo</i>","abstract":"Appropriate development of long-range nervous system circuitry requires dynamic regulation of subcellular mRNA localization, but little is known about how specific neuron subtypes control this critical process in vivo . Here, we employ an integrated genetic and temporally controlled approach to investigate in vivo developmental mRNA dynamics in somata and axons of a prototypical cerebral cortex projection neuron subtype, callosal projection neurons (CPN), which connect the cortical hemispheres to integrate sensorimotor information, and regulate high-level associative cognition and behavior. We identify cis-regulatory elements that are associated with mRNA turnover in CPN somata, including specific 3’ untranslated regions (UTRs), and elucidate distinct modes of axon transport in CPN axons for function-specific mRNA classes. Together, these findings elucidate how developing CPN control subcellular mRNA localization, and how dysregulation of these processes might lead to neurodevelopmental and/or neurodegenerative disorders. More broadly, this work identifies general mRNA localization mechanisms that likely function across projection neuron subtypes, and in other polarized cells.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":556185,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":733668,"name":"Dustin E. Tillman","orcid":"0000-0002-7450-0927","position":1,"is_corresponding":false},{"id":1455908,"name":"Melody I. Ross","orcid":null,"position":2,"is_corresponding":false},{"id":481647,"name":"Jeffrey D. Macklis","orcid":"0000-0003-3662-9698","position":3,"is_corresponding":false},{"id":1161978,"name":"Priya Veeraraghavan","orcid":null,"position":0,"is_corresponding":true}],"reference_count":98,"raw_metadata":null,"created_at":"2026-07-19T02:55:08.896385Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}