{"doi":"10.1101/2025.04.23.648848","title":"<sup>2</sup> H MRI-based quantification of leucine uptake in glioblastoma multiforme","abstract":"Abstract Glioblastoma (GBM) brain tumors are among the most lethal of all human cancers, with a median survival time of ∼15 months. Treatment planning requires radiologic demarcation of tumor boundaries with contrast-enhanced magnetic resonance imaging (CE MRI); however, significant tumor burden extends beyond the contrast-enhancing margins of the tumor. GBM tumors have an increased expression of amino acid (AA) transporters, including the Alanine, Serine, Cysteine Transporter 2 (ASCT2) and the L-Type Amino Acid Transporter 1 (LAT1). This upregulation has been leveraged in positron emission tomography (PET) studies to detect tumor burden beyond the contrast-enhancing margins identified by standard-of-care CE MRI. Here we leverage recent approaches in deuterium metabolic magnetic resonance with this known upregulation of AA transporters in GBM to demonstrate that 2 H MR can detect glioma based on enhanced branched- chain amino acid (BCAA) uptake. To the best of our knowledge, these data represent the first non- invasive quantification of AA concentrations in brain tumor and raises the potential to (i) detect tumor burden beyond contrast-enhancing margins and (ii) quantify AA metabolism using 2 H MR spectroscopy.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":564774,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":559862,"name":"Xia Ge","orcid":null,"position":1,"is_corresponding":false},{"id":903147,"name":"Kyu-Ho Song","orcid":"0000-0002-9949-3022","position":2,"is_corresponding":false},{"id":1260721,"name":"Dustin Grief","orcid":null,"position":3,"is_corresponding":false},{"id":341471,"name":"Shannon P. Fortin Ensign","orcid":"0000-0002-7433-528X","position":4,"is_corresponding":false},{"id":410980,"name":"Vikram D. Kodibagkar","orcid":"0000-0003-1933-8821","position":5,"is_corresponding":false},{"id":247456,"name":"Leland Hu","orcid":"0000-0001-9282-7619","position":6,"is_corresponding":false},{"id":323929,"name":"Joel R. Garbow","orcid":"0000-0002-0654-2703","position":7,"is_corresponding":false},{"id":267702,"name":"Scott C. Beeman","orcid":"0000-0001-6721-1185","position":8,"is_corresponding":false},{"id":1403683,"name":"Samantha McClendon","orcid":null,"position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:56:24.872312Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}