{"doi":"10.1101/2025.04.22.650109","title":"Mass spectrometry-based quantification of proteins and post-translational modifications in dried blood: longitudinal sampling of patients with sepsis in Tanzania","abstract":"The proteomic analysis of blood is routine for disease phenotyping and biomarker development. Whole blood is commonly separated into soluble and cellular fractions. However, this can introduce pre-analytical variability; and analysis of a single component (which is common) may ignore important pathophysiology. We have recently developed methods for the facile processing of dried blood for mass spectrometry-based quantification of the proteome, N-glycoproteome and phosphoproteome. Here, we applied this approach to 38 patients in Tanzania who presented to the hospital with sepsis. Blood was collected on Mitra devices at presentation and 1, 3 and 28-42 days post-enrollment. Processing of 96 total samples was performed in plate-based formats and completed within two days. Approximately 2,000 protein groups and 8,000 post-translational modifications were quantified in 3 LC-MS/MS runs at ~1.5 hours per sample. Analysis of differential abundance revealed blood proteome signatures of acute phase response and neutrophilic inflammation that partially resolved at the 28-42 day timepoint. Numerous analytes correlated with clinical laboratory values for c-reactive protein and white blood cell counts, as well as the Universal Vital Assessment illness severity score. These datasets serve as proof-of-concept for large scale MS-based (sub)phenotyping of disease using dried blood and are available via the ProteomeXchange consortium (PXD060377).","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":556210,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9458,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":690715,"name":"T. McMahon","orcid":"0000-0002-3404-3223","position":1,"is_corresponding":false},{"id":376443,"name":"James S. Ngocho","orcid":"0000-0001-6242-9863","position":2,"is_corresponding":false},{"id":1289287,"name":"Asia Hemed Kipengele","orcid":null,"position":3,"is_corresponding":false},{"id":1055286,"name":"Marlene Violette","orcid":"0000-0001-9351-3996","position":4,"is_corresponding":false},{"id":724086,"name":"Youwei Chen","orcid":"0000-0002-5663-6118","position":5,"is_corresponding":false},{"id":478837,"name":"Deng B. Madut","orcid":"0000-0003-4023-3928","position":6,"is_corresponding":false},{"id":1023254,"name":"Robert S. Plumb","orcid":"0000-0002-1380-9285","position":7,"is_corresponding":false},{"id":1455530,"name":"Andrea Li Ann Wong","orcid":"0000-0002-5860-7847","position":8,"is_corresponding":false},{"id":572260,"name":"Lingye Chen","orcid":"0000-0002-9877-2118","position":9,"is_corresponding":false},{"id":225720,"name":"Grace M. Lee","orcid":"0000-0002-0051-2466","position":10,"is_corresponding":false},{"id":954549,"name":"Philoteus A. Sakasaka","orcid":null,"position":11,"is_corresponding":false},{"id":341832,"name":"Blandina T. Mmbaga","orcid":"0000-0002-5550-1916","position":12,"is_corresponding":false},{"id":22443,"name":"John A. Crump","orcid":"0000-0002-4529-102X","position":13,"is_corresponding":false},{"id":355859,"name":"Micah T. McClain","orcid":"0000-0001-8034-1136","position":14,"is_corresponding":false},{"id":33039,"name":"Christopher W. Woods","orcid":"0000-0001-7240-2453","position":15,"is_corresponding":false},{"id":345414,"name":"Venance P. Maro","orcid":"0000-0003-3838-0312","position":16,"is_corresponding":false},{"id":345416,"name":"Matthew P. Rubach","orcid":"0000-0001-5262-1421","position":17,"is_corresponding":false},{"id":364137,"name":"Matthew W. Foster","orcid":"0000-0003-0212-2346","position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-19T02:55:08.896385Z","pmid":"40492198","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}