{"doi":"10.1101/2025.04.17.649293","title":"High-performance genetically-encoded green and red fluorescent biosensors for pyruvate","abstract":"Abstract Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F 0 > 10) single fluorescent protein (FP)-based biosensors has hindered efforts to investigate the physiological role of pyruvate. Here, we present the GreenPy1 and ApplePy1 series, which are green FP (GFP)-based and red FP (RFP)-based pyruvate biosensors, respectively. Both series exhibit large fluorescence intensity change (Δ F / F 0 ∼ 20 to >40) and a range of affinities (10s of μM to several mM). We demonstrate the utility of these pyruvate biosensors for multicolor imaging of metabolite concentration changes in mammalian cells.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":556169,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.945,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1211048,"name":"Saaya Hario","orcid":"0000-0003-0127-3142","position":1,"is_corresponding":false},{"id":1455890,"name":"Carl Suerte","orcid":null,"position":2,"is_corresponding":false},{"id":1455891,"name":"Issei Yamaguchi","orcid":null,"position":3,"is_corresponding":false},{"id":1368777,"name":"Kenryo Sakoi","orcid":null,"position":4,"is_corresponding":false},{"id":1455489,"name":"Adam Thuen","orcid":"0000-0002-5235-5466","position":5,"is_corresponding":false},{"id":1455892,"name":"Brekken Meznarich","orcid":null,"position":6,"is_corresponding":false},{"id":275972,"name":"Mikhail Drobizhev","orcid":"0000-0001-9072-1300","position":7,"is_corresponding":false},{"id":1211050,"name":"Takuya Terai","orcid":"0000-0002-3425-3589","position":8,"is_corresponding":false},{"id":1408098,"name":"Kei Takahashi","orcid":"0000-0002-7925-6636","position":9,"is_corresponding":false},{"id":197736,"name":"Robert E Campbell","orcid":"0000-0003-0604-092X","position":10,"is_corresponding":false},{"id":1226006,"name":"Shosei Imai","orcid":"0000-0003-0388-4764","position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":null,"created_at":"2026-07-19T02:55:08.896385Z","pmid":"40631294","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}