{"doi":"10.1101/2025.01.28.635394","title":"CPSF6 Promotes HIV-1 Preintegration Complex Function","abstract":"ABSTRACT Cleavage and polyadenylation specificity factor 6 (CPSF6) is part of the cellular cleavage factor I mammalian (CFIm) complex that regulates mRNA processing and polyadenylation. CPSF6 also functions as a HIV-1 capsid (CA) binding host factor and promotes viral DNA integration targeting into gene dense regions of the host genome. However, the effects of CPSF6 on the activity of the HIV-1 preintegration complex (PIC) - the machinery that carries out viral DNA integration to establish infection - is unknown. To study CPSF6’s role in HIV-1 PIC function, we extracted PICs from cells depleted of CPSF6 or expressing a CPSF6 mutant that cannot bind to CA. These PICs exhibited significantly lower integration activity when compared to the control PICs. Addition of recombinant CPSF6 restored the integration activity of PICs extracted from the mutant cells, suggesting a direct role of CPSF6 in PIC function. To solidify CPSF6’s effect on PIC function, we inoculated CPSF6-depleted and CPSF6-mutant cells with HIV-1 particles and measured viral DNA integration into the host genome. A significant reduction in viral integration in these cells was detected and this defect was not a consequence of reduced reverse transcription or nuclear entry. Additionally, mutant viruses deficient in CA-CPSF6 binding showed no integration defect in CPSF6 mutant cells. Finally, sequencing analysis revealed that HIV-1 integration in the CPSF6 mutant cells was significantly redirected from the gene dense regions of the host genome. Collectively, these results suggest that CPSF6-CA interaction regulates PIC function both in vitro and in infected cells. IMPORTANCE HIV-1 infection is dependent on the interaction of the virus with host factors. However, the molecular details of virus-host factor interactions are not fully understood. For instance, HIV-1 capsid provides binding interfaces for several host factors. CPSF6 is one such capsid-binding host factor, whose cellular function is to regulate mRNA processing and polyadenylation. Initial work identified a truncated cytosolic form of CPSF6 that restricted HIV infection by blocking viral nuclear entry. However, it is now established that the full-length CPSF6 primarily promotes integration targeting into gene dense regions of the host genome. Here we report that CPSF6-CA interaction promotes the activity of HIV-1 preintegration complexes (PICs). We also observed that disruption of CPSF6-CA binding in target cells significantly reduced viral integration and directed integration targeting away from gene-dense regions. These findings demonstrate a critical role for the CPSF6-CA interaction in PIC function and integration targeting.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":561065,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9514,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":561574,"name":"Sooin Jang","orcid":"0000-0003-2937-0948","position":1,"is_corresponding":false},{"id":1253909,"name":"Rajasree Chakraborty","orcid":"0000-0001-8704-9648","position":2,"is_corresponding":false},{"id":1218560,"name":"Rajalingam Radhakrishnan","orcid":"0009-0004-6344-903X","position":3,"is_corresponding":false},{"id":1219141,"name":"Bjarki Arnarson","orcid":null,"position":4,"is_corresponding":false},{"id":721738,"name":"Prem Prakash","orcid":"0000-0001-6507-7875","position":5,"is_corresponding":false},{"id":843869,"name":"Daphne Cornish","orcid":"0000-0003-3835-5694","position":6,"is_corresponding":false},{"id":1409523,"name":"Nicholas Rohlfes","orcid":null,"position":7,"is_corresponding":false},{"id":253444,"name":"Parmit K. Singh","orcid":"0000-0002-9657-2526","position":8,"is_corresponding":false},{"id":298945,"name":"Jiong Shi","orcid":"0000-0001-7548-7616","position":9,"is_corresponding":false},{"id":307290,"name":"Christopher Aiken","orcid":"0000-0002-2476-4078","position":10,"is_corresponding":false},{"id":329247,"name":"Edward M. Campbell","orcid":"0000-0001-5665-9195","position":11,"is_corresponding":false},{"id":36741,"name":"Judd F. Hultquist","orcid":"0000-0001-6424-4280","position":12,"is_corresponding":false},{"id":228712,"name":"Alan Engelman","orcid":"0000-0002-9709-2591","position":13,"is_corresponding":false},{"id":1412030,"name":"Muthukumar Balsubramaniam","orcid":null,"position":14,"is_corresponding":false},{"id":374805,"name":"Chandravanu Dash","orcid":"0000-0003-4466-8355","position":15,"is_corresponding":false},{"id":374798,"name":"Evan Chaudhuri","orcid":"0000-0003-2319-8657","position":0,"is_corresponding":true}],"reference_count":136,"raw_metadata":null,"created_at":"2026-07-19T02:55:46.921885Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}